PF3D7_0100100 (VAR)

Genome location: Pf3D7_01_v3:29,510..37,126(+)

Genome classification: SubtelomericHypervariable

Function and Localization

Product Description: erythrocyte membrane protein 1, PfEMP1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020002	host cell plasma membrane
Component	GO:0020030	infected host cell surface knob
Function	GO:0050839	cell adhesion molecule binding
Process	GO:0020033	antigenic variation
Process	GO:0098609	cell-cell adhesion
Process	GO:0020035	cytoadherence to microvasculature, mediated by symbiont protein
Process	GO:0020013	modulation by symbiont of host erythrocyte aggregation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.02	0.99
Trophozoite	possibly expressed	0.02	0.99
Schizont	expressed	0.07	0.94
Gametocyte	expressed	0.04	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0100100

Resistome Missense Mutations: I119N, C294R, K310E, K341E, K344I, N345K, G349E, E1214G, G1471A, Q1478H, D1482E, T1856I, T1857P, A1858T, N1883D, K1903N, E2047K, A2050D, N2133D, R2139K

Resistome Compounds with Missense Mutations: AN13762, AN13956, Atovaquone, GNF-Pf-5611, Halofuginone, KAE678/NITD678, MMV019066, OSM-S-106

Resistome # Samples with Disruptive Mutations: 19 (18 missense, 10 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.706 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9NFB6

"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=1.96) with 5MZA (Global RMSD=4.45)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104345

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 2687

Non-coding: 474 | Synonymous: 648 | Nonsynonymous: 1546 | Stop Codon: 19

Protein Information

Protein Length: 2163 | Molecular Weight (kDa): 245.807

UniProt ID(s): A0A5P1A0A2, A0A5P1A1E9, A0A5P1A1X2, A0A5P1A1Y7, A0A5P1A384, B5LGG5, Q9NFB6, T1Q3H8

PDB ID(s): None

Isoelectric Point: 5.24

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004258	Duffy-binding-like domain
InterPro	IPR008602	Duffy-antigen binding
InterPro	IPR029210	Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment
InterPro	IPR029211	Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment
InterPro	IPR041480	Cysteine-rich interdomain region 1 gamma
PFam	PF03011	Duffy-binding-like domain
PFam	PF05424	Duffy-antigen binding
PFam	PF15445	Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment
PFam	PF15447	Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment
PFam	PF18562	Cysteine-rich interdomain region 1 gamma
Superfamily	SSF140924	N/A