PF3D7_0100300 (VAR)

Genome location: Pf3D7_01_v3:42,367..46,507(-)

Genome classification: SubtelomericHypervariable

Function and Localization

Product Description: erythrocyte membrane protein 1, PfEMP1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.00	1.00
Trophozoite	possibly expressed	0.00	1.00
Schizont	possibly expressed	0.00	1.00
Gametocyte	possibly expressed	0.00	1.00

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0100300

Resistome Missense Mutations: Y137F, N145H, H147D, V1222I

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.663 | MFS: -2.461 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9NFB4

"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=0.28) with 5MZA (Global RMSD=4.83)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104345

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 1093

Non-coding: 21 | Synonymous: 414 | Nonsynonymous: 641 | Stop Codon: 17

Protein Information

Protein Length: 1327 | Molecular Weight (kDa): 153.32

UniProt ID(s): A0A0F6P8H0, A0A5P1A0B7, A0A5P1A0V6, A0A5P1A1A1, A0A5P1A1D6, A0A5P1A1K6, A0A5P1A1L4, A0A5P1A2K8, A1KQT3, A3R6U7, Q1KV45, Q1KV46, Q1KVF5, Q9NFB4

PDB ID(s): None

Isoelectric Point: 5.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008602	Duffy-antigen binding
InterPro	IPR029210	Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment
InterPro	IPR029211	Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment
PFam	PF05424	Duffy-antigen binding
PFam	PF15445	Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment
PFam	PF15447	Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment
Superfamily	SSF140924	N/A