About Gene List

PF3D7_0102500 (EBA181)

Genome location: Pf3D7_01_v3:109,005..117,516(-)

Genome classification: Core

Function and Localization

Product Description: erythrocyte binding antigen-181

SignalP Peptide: MKGKMNMCLFFFYSILYVVLCTYVLG

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:1903561 extracellular vesicle
Component GO:0020009 microneme
Function GO:0008201 heparin binding
Function GO:0033691 sialic acid binding
Process GO:0098609 cell-cell adhesion
Process GO:0044409 entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.68 0.80
Trophozoite possibly expressed 0.09 0.95
Schizont expressed 0.47 0.85
Gametocyte possibly expressed 0.05 0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0102500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -2.102 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1332700)

  • Relative Growth Rate: 0.17 ± 0.11
  • Confidence: 5.74

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2B4

"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=0.31) with 5MZA (Global RMSD=4.70)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_160717

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 54 16 83
disruptive 69 195 90 222
missense 54 150 78 176

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 18 85 48 77
disruptive 110 379 146 301
missense 79 281 124 219

PlasmoDB Total SNPs: 369

Non-coding: 248 | Synonymous: 55 | Nonsynonymous: 66 | Stop Codon: 0

Protein Information

Protein Length: 1567 | Molecular Weight (kDa): 181.15

UniProt ID(s): A0A0K2RVP3, A0A0K2RVP5, A0A0K2RVP8, A0A0K2RVS8, A0A0K2RWA2, A0A0K2RWA7, Q105Z6, Q105Z8, Q106B2, Q6RI89, Q6RKB5, Q6RKB6, Q6RKB8, Q6RKC0, Q6RKC1, Q6RKC2, Q6RKC3, Q6RKC4, Q8I2B4, Q8IAD5, Q8IAD6

PDB ID(s): None

Isoelectric Point: 4.71

Protein Domain Annotations:

Source Family ID Description
InterPro IPR008602 Duffy-antigen binding
InterPro IPR021620 Erythrocyte binding antigen 175, C-terminal
PFam PF05424 Duffy-antigen binding
PFam PF11556 Erythrocyte binding antigen 175, C-terminal
Superfamily SSF140924 N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
12556470 A novel erythrocyte binding antigen-175 paralogue from Plasmodium falciparumdefines a new trypsin-resistant receptor on human erythrocytes. Gilberger TW, Thompson JK, ..., Cowman AF 10.1074/jbc.M211446200
15477199 Myosin-like sequences in the malaria parasite Plasmodium falciparum bind humanerythrocyte membrane protein 4.1. Lanzillotti R, Coetzer TL https://pubmed.ncbi.nlm.nih.gov/15477199/
24212193 Analyses of interactions between heparin and the apical surface proteins ofPlasmodium falciparum. Kobayashi K, Takano R, ..., Kato K 10.1038/srep03178
28944300 Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. Abdi A, Yu L, ..., Rayner J 10.12688/wellcomeopenres.11910.2
25288451 Intrinsic disorder within the erythrocyte binding-like proteins from Plasmodium falciparum Blanc M, Coetzer TL, ..., Jensen MR 10.1016/j.bbapap.2014.09.023
23118907 Plasmodium falciparum field isolates from South America use an atypical red blood cell invasion pathway associated with invasion ligand polymorphisms Lopez-Perez M, Villasis E, ..., Lustigman S 10.1371/journal.pone.0047913
27427762 Neutralizing Antibodies against Plasmodium falciparum Associated with Successful Cure after Drug Therapy Goh YS, Peng K, ..., Renia L 10.1371/journal.pone.0159347
28787025 Acquisition, maintenance and adaptation of invasion inhibitory antibodies against Plasmodium falciparum invasion ligands involved in immune evasion Tijani MK, Babalola OA, ..., Persson KEM 10.1371/journal.pone.0182187
37653544 Recent increase in low complexity polygenomic infections and sialicacid-independent invasion pathways in Plasmodium falciparum from Western Gambia Nganyewo NN, Bojang F, ..., Amambua-Ngwa A 10.1186/s13071-023-05929-4
21160096 [Proteins involved in invasion of human red blood cells by malaria parasites] Jaskiewicz E, Graczyk J, Rydzak J https://pubmed.ncbi.nlm.nih.gov/21160096/
20843207 Association between naturally acquired antibodies to erythrocyte-binding antigens of Plasmodium falciparum and protection from malaria and high-densityparasitemia Richards JS, Stanisic DI, ..., Beeson JG 10.1086/656413
20050806 Erythrocyte invasion and merozoite ligand gene expression in severe and mildPlasmodium falciparum malaria Gomez-Escobar N, Amambua-Ngwa A, ..., Conway DJ 10.1086/649902
17984363 Differential antibody responses to Plasmodium falciparum invasion ligand proteinsin individuals living in malaria-endemic areas in Brazil and Cameroon Ford L, Lobo CA, ..., Lustigman S https://pubmed.ncbi.nlm.nih.gov/17984363/
23110555 Anti-Plasmodium falciparum invasion ligand antibodies in a low malaria transmission region, Loreto, Peru Villasis E, Lopez-Perez M, ..., Lustigman S 10.1186/1475-2875-11-361
19421327 Case-control approach to identify Plasmodium falciparum polymorphisms associated with severe malaria Chokejindachai W, Conway DJ 10.1371/journal.pone.0005454
25870227 Variation in Plasmodium falciparum erythrocyte invasion phenotypes and merozoite ligand gene expression across different populations in areas of malaria endemicity Bowyer PW, Stewart LB, ..., Conway DJ 10.1128/IAI.03009-14
36812236 Effects of anti-malarial prophylaxes on maternal transfer of Immunoglobulin-G(IgG) and association to immunity against Plasmodium falciparum infections amongchildren in a Ugandan birth cohort Okek EJ, Ocan M, ..., Kamya MR 10.1371/journal.pone.0277789
31234897 Erythrocyte glycophorins as receptors for Plasmodium merozoites Jaskiewicz E, Jodlowska M, Kaczmarek R, Zerka A 10.1186/s13071-019-3575-8
23740926 Acquired antibodies to merozoite antigens in children from Uganda withuncomplicated or severe Plasmodium falciparum malaria Ahmed Ismail H, Ribacke U, ..., Persson KE 10.1128/CVI.00156-13
23776178 Erythrocyte-binding antigens of Plasmodium falciparum are targets of humaninhibitory antibodies and function to evade naturally acquired immunity Persson KE, Fowkes FJ, ..., Beeson JG 10.4049/jimmunol.1300444
21149582 Reticulocyte and erythrocyte binding-like proteins function cooperatively ininvasion of human erythrocytes by malaria parasites Lopaticki S, Maier AG, ..., Cowman AF 10.1128/IAI.01021-10
24834503 Patterns of selection on Plasmodium falciparum erythrocyte-binding antigens after the colonization of the New World Yalcindag E, Rougeron V, ..., Prugnolle F 10.1111/mec.12696
35637187 Stochastic expression of invasion genes in Plasmodium falciparum schizonts Tripathi J, Zhu L, ..., Bozdech Z 10.1038/s41467-022-30605-z
19204093 Polymorphisms in erythrocyte binding antigens 140 and 181 affect function and binding but not receptor specificity in Plasmodium falciparum Maier AG, Baum J, ..., Cowman AF 10.1128/IAI.01331-08