Product Description: aspartate--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.12 (Aspartate--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0017101 | aminoacyl-tRNA synthetase multienzyme complex |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0004815 | aspartate-tRNA ligase activity |
| Function | GO:0042803 | protein homodimerization activity |
| Process | GO:0006422 | aspartyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.56 | 0.74 |
| Trophozoite | expressed | 1.42 | 0.35 |
| Schizont | expressed | 0.21 | 0.87 |
| Gametocyte | expressed | 0.32 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0102900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -2.954 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0210200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2B1
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.39) with 3REX (Global RMSD=9.37)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.12 | aspartate-tRNA ligase | KClNH4+tRNANO3-IO3-CNS-RNAiCaCl2NiCl2ClO3-K2HPO4CH3COO-... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100778
Most Similar Human Ortholog: H7BZ35
TM-align score: 0.94 | RMSD: 1.73
Seq Identity: 0.49 | Length: 187 / 626
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000115866 | aspartyl-tRNA synthetase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 40 | 24 | 28 |
| disruptive | 7 | 46 | 23 | 71 |
| missense | 7 | 46 | 23 | 64 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 65 | 25 | 30 |
| disruptive | 14 | 120 | 54 | 134 |
| missense | 13 | 102 | 46 | 99 |
PlasmoDB Total SNPs: 88
Non-coding: 57 | Synonymous: 22 | Nonsynonymous: 9 | Stop Codon: 0
Protein Length: 626 | Molecular Weight (kDa): 73.45
UniProt ID(s): Q8I2B1
PDB ID(s): None
Isoelectric Point: 8.25
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002312 | Aspartyl/Asparaginyl-tRNA synthetase, class IIb |
| InterPro | IPR004364 | Aminoacyl-tRNA synthetase, class II (D/K/N) |
| InterPro | IPR004365 | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type |
| InterPro | IPR004523 | Aspartate-tRNA synthetase, type 2 |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| PFam | PF00152 | Aminoacyl-tRNA synthetase, class II (D/K/N) |
| PFam | PF01336 | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF55681 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 19443655 | Plasmodial aspartyl-tRNA synthetases and peculiarities in Plasmodium falciparum. | Bour T, Akaddar A, ..., Frugier M | 10.1074/jbc.M109.015297 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |