About Gene List

PF3D7_0103100 (VPS51)

Genome location: Pf3D7_01_v3:134,155..140,371(-)

Genome classification: Core

Function and Localization

Product Description: vacuolar protein sorting-associated protein 51, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:1990745 EARP complex
Component GO:0000938 GARP complex
Component GO:0005794 Golgi apparatus
Component GO:0016020 membrane
Process GO:0007030 Golgi organization
Process GO:0048193 Golgi vesicle transport
Process GO:0032456 endocytic recycling
Process GO:0007041 lysosomal transport
Process GO:0015031 protein transport
Process GO:0042147 retrograde transport, endosome to Golgi

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.07 0.97
Trophozoite possibly expressed 0.36 0.78
Schizont possibly expressed 0.36 0.77
Gametocyte expressed 0.47 0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0103100

Resistome Missense Mutations: K922N, D936H

Resistome Compounds with Missense Mutations: GNF707, MMV019017, MMV688533

Resistome # Samples with Disruptive Mutations: 7 (7 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.165 | MFS: -2.812 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2A9

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=2.61) with 6JWT (Global RMSD=17.37)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103074

Most Similar Human Ortholog: E9PMB6

TM-align score: 0.59 | RMSD: 3.50

Seq Identity: 0.12 | Length: 99 / 1634

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000149823 VPS51 subunit of GARP complex

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 26 75 34 87
disruptive 71 270 155 259
missense 40 208 135 223

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 36 145 57 93
disruptive 124 496 222 347
missense 72 387 196 242

PlasmoDB Total SNPs: 347

Non-coding: 103 | Synonymous: 128 | Nonsynonymous: 116 | Stop Codon: 0

Protein Information

Protein Length: 1634 | Molecular Weight (kDa): 197.115

UniProt ID(s): Q8I2A9

PDB ID(s): None

Isoelectric Point: 6.71

Protein Domain Annotations:

Source Family ID Description
InterPro IPR014812 Vacuolar protein sorting-associated protein 51
PFam PF08700 N/A
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
38055775 GARP and EARP are required for efficient BoHV-1 replication as identified by agenome wide CRISPR knockout screen Tan WS, Rong E, ..., Dalziel RG 10.1371/journal.ppat.1011822