PF3D7_0103200 (NT4)

Genome location: Pf3D7_01_v3:140,494..143,049(-)

Genome classification: Core

Function and Localization

Product Description: nucleoside transporter 4

SignalP Peptide: N/A

# Transmembrane Domains: 11

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016020	membrane
Function	GO:0005345	purine nucleobase transmembrane transporter activity
Function	GO:0015211	purine nucleoside transmembrane transporter activity
Process	GO:0006863	purine nucleobase transport
Process	GO:0015860	purine nucleoside transmembrane transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.18	0.91
Trophozoite	expressed	0.72	0.60
Schizont	expressed	0.16	0.89
Gametocyte	expressed	0.57	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0103200

Resistome Missense Mutations: S22C, H26Q, H320L

Resistome Compounds with Missense Mutations: OSM-S-106

Resistome # Samples with Disruptive Mutations: 8 (8 missense, 8 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.064 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0209900)

Relative Growth Rate: 1.03 ± 0.08
Confidence: 6.45

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0209900)

Modification: Disrupted | RMgm-5234

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2A8

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533310

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	21	13	30
disruptive	10	28	9	26
missense	10	28	8	24

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	47	26	39
disruptive	20	49	35	62
missense	17	39	24	53

PlasmoDB Total SNPs: 208

Non-coding: 178 | Synonymous: 15 | Nonsynonymous: 15 | Stop Codon: 0

Protein Information

Protein Length: 434 | Molecular Weight (kDa): 50.011

UniProt ID(s): Q8I2A8

PDB ID(s): None

Isoelectric Point: 8.86

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR036259	MFS transporter superfamily
PFam	N/A	N/A
Superfamily	SSF103473	MFS transporter superfamily

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
22670848	Malaria parasite type 4 equilibrative nucleoside transporters (ENT4) are purinetransporters with distinct substrate specificity.	Frame IJ, Merino EF, ..., Akabas MH	10.1042/BJ20112220