PF3D7_0103400

Genome location: Pf3D7_01_v3:147,031..152,795(-)

Genome classification: Core

Function and Localization

Product Description: zinc-carboxypeptidase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.17.10 (Carboxypeptidase E)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.92	0.65
Trophozoite	expressed	1.56	0.29
Schizont	expressed	1.64	0.27
Gametocyte	expressed	1.67	0.25

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0103400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.537 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0209700)

Relative Growth Rate: 0.99 ± 0.08
Confidence: 6.36

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0209700)

Modification: Disrupted | RMgm-1708

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.17.10	carboxypeptidase E	EDTAHgCl2CuCl2FeSO4CdCl2lysinearginineOxytoxinSubstance PVasopressinMet-enkephalinLeu-enkephalin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101273

Most Similar Human Ortholog: F8W7R4

TM-align score: 0.59 | RMSD: 3.89

Seq Identity: 0.31 | Length: 458 / 1620

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000135049	ATP/GTP binding protein 1
ENSG00000146856	ATP/GTP binding protein like 3
ENSG00000165923	ATP/GTP binding protein like 2
ENSG00000273540	ATP/GTP binding protein like 1
ENSG00000285501	ATP/GTP binding protein like 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	20	90	48	62
disruptive	119	303	136	315
missense	65	224	116	247

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	31	157	54	101
disruptive	175	556	200	353
missense	89	428	160	233

PlasmoDB Total SNPs: 305

Non-coding: 60 | Synonymous: 144 | Nonsynonymous: 101 | Stop Codon: 0

Protein Information

Protein Length: 1620 | Molecular Weight (kDa): 191.963

UniProt ID(s): None

PDB ID(s): None

Isoelectric Point: 8.79

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	SSF53187	N/A
Superfamily	SSF58104