Product Description: ATP-dependent DNA/RNA helicase PSH1
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005730 | nucleolus |
| Component | GO:0005634 | nucleus |
| Function | GO:0043138 | 3'-5' DNA helicase activity |
| Function | GO:0043139 | 5'-3' DNA helicase activity |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003724 | RNA helicase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.19 | 0.91 |
| Trophozoite | expressed | 0.85 | 0.56 |
| Schizont | expressed | 0.31 | 0.82 |
| Gametocyte | expressed | 1.05 | 0.48 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0103600
Resistome Missense Mutations: H144N, R1234K
Resistome Compounds with Missense Mutations: MMV665794
Resistome # Samples with Disruptive Mutations: 4 (4 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.61 | #Insertions: 2
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2A4
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.08) with 3RRM (Global RMSD=8.03)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 64 | 41 | 92 |
| disruptive | 102 | 249 | 111 | 304 |
| missense | 55 | 168 | 84 | 230 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 147 | 59 | 99 |
| disruptive | 204 | 507 | 200 | 425 |
| missense | 89 | 357 | 156 | 271 |
PlasmoDB Total SNPs: 418
Non-coding: 158 | Synonymous: 145 | Nonsynonymous: 113 | Stop Codon: 2
Protein Length: 1534 | Molecular Weight (kDa): 183.358
UniProt ID(s): Q8I2A4
PDB ID(s): None
Isoelectric Point: 10.15
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 31469232 | Biochemical characterization of Plasmodium falciparum parasite specific helicase1 (PfPSH1). | Chauhan M, Sourabh S, ..., Tuteja R | 10.1002/2211-5463.12728 |
| 30885288 | N-terminal formylmethionine as a novel initiator and N-degron of eukaryoticproteins | Kim JM | 10.5483/BMBRep.2019.52.3.069 |
| 27794026 | Histone H4 Facilitates the Proteolysis of the Budding Yeast CENP-ACse4Centromeric Histone Variant | Deyter GM, Hildebrand EM, Barber AD, Biggins S | 10.1534/genetics.116.194027 |
| 35234920 | Cdc48Ufd1/Npl4 segregase removes mislocalized centromeric histone H3 variantCENP-A from non-centromeric chromatin | Ohkuni K, Gliford L, ..., Basrai MA | 10.1093/nar/gkac135 |
| 26990181 | Histone chaperones FACT and Spt6 prevent histone variants from turning into histone deviants | Jeronimo C, Robert F | 10.1002/bies.201500122 |
| 32111629 | Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-Terminal Sumoylation | Ohkuni K, Suva E, ..., Basrai MA | 10.1534/genetics.120.303090 |
| 30077637 | Ubiquitin protease Ubp8 is necessary for S. cerevisiae respiration | Leo M, Fanelli G, ..., Filetici P | 10.1016/j.bbamcr.2018.07.025 |
| 33751052 | Reduced gene dosage of histone H4 prevents CENP-A mislocalization and chromosomalinstability in Saccharomyces cerevisiae | Eisenstatt JR, Ohkuni K, ..., Basrai MA | 10.1093/genetics/iyab033 |
| 38248957 | The Protein Response of Salt-Tolerant Zygosaccharomyces rouxii toHigh-Temperature Stress during the Lag Phase | Hu N, Xiao X, ..., Li X | 10.3390/jof10010048 |
| 29522205 | Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition intochromatin in budding yeast | Hewawasam GS, Dhatchinamoorthy K, ..., Gerton JL | 10.1093/nar/gky169 |
| 28928274 | The Ubiquitin Ligase (E3) Psh1p Is Required for Proper Segregation of bothCentromeric and Two-Micron Plasmids in Saccharomyces cerevisiae | Metzger MB, Scales JL, Dunklebarger MF, Weissman AM | 10.1534/g3.117.300227 |
| 29432128 | N-terminal Sumoylation of Centromeric Histone H3 Variant Cse4 Regulates ItsProteolysis To Prevent Mislocalization to Non-centromeric Chromatin | Ohkuni K, Levy-Myers R, ..., Basrai MA | 10.1534/g3.117.300419 |
| 34217622 | Molecular basis for the selective recognition and ubiquitination of centromerichistone H3 by yeast E3 ligase Psh1 | Zhou N, Shi L, Shan S, Zhou Z | 10.1016/j.jgg.2021.04.007 |
| 32295767 | Dbf4-Dependent Kinase (DDK)-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A in Saccharomyces cerevisiae | Eisenstatt JR, Boeckmann L, ..., Basrai MA | 10.1534/g3.120.401131 |
| 30012561 | A Genome-Wide Screen Reveals a Role for the HIR Histone Chaperone Complex inPreventing Mislocalization of Budding Yeast CENP-A | Ciftci-Yilmaz S, Au WC, ..., Basrai MA | 10.1534/genetics.118.301305 |
| 28158539 | Defects in methylation of arginine 37 on CENP-A/Cse4 are compensated by theubiquitin ligase complex Ubr2/Mub1 | Samel A, Nguyen TKL, Ehrenhofer-Murray AE | 10.1093/femsyr/fox009 |
| 28819127 | Multiple E3s promote the degradation of histone H3 variant Cse4 | Cheng H, Bao X, ..., Rao H | 10.1038/s41598-017-08923-w |