Genome location: Pf3D7_01_v3:172,470..175,055(+)
Genome classification: Core
Product Description: parasite-infected erythrocyte surface protein
SignalP Peptide: MRKTKGRLVFYLCISIWCLFVNICKNSDT
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0030134 | COPII-coated ER to Golgi transport vesicle |
| Component | GO:0000139 | Golgi membrane |
| Component | GO:0005789 | endoplasmic reticulum membrane |
| Component | GO:0005793 | endoplasmic reticulum-Golgi intermediate compartment |
| Function | GO:0005537 | mannose binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0007030 | Golgi organization |
| Process | GO:0007029 | endoplasmic reticulum organization |
| Process | GO:0006888 | endoplasmic reticulum to Golgi vesicle-mediated transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.80 | 0.57 |
| Schizont | expressed | 0.19 | 0.88 |
| Gametocyte | expressed | 0.32 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0103900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.128 | MFS: -3.12 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0209200)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0209200)
Modification: Disrupted | RMgm-4959
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2A1
"Best" AlphaFill ligand hit: SFG (sinefungin, Local RMSD=6.06) with 3BBH (Global RMSD=21.47)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101728
Most Similar Human Ortholog: D6RIU4
TM-align score: 0.80 | RMSD: 2.47
Seq Identity: 0.17 | Length: 173 / 575
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000114988 | lectin, mannose binding 2 like |
| ENSG00000169223 | lectin, mannose binding 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 30 | 15 | 47 |
| disruptive | 15 | 43 | 34 | 75 |
| missense | 5 | 33 | 31 | 59 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 61 | 26 | 45 |
| disruptive | 26 | 127 | 56 | 134 |
| missense | 11 | 95 | 45 | 101 |
PlasmoDB Total SNPs: 192
Non-coding: 156 | Synonymous: 24 | Nonsynonymous: 11 | Stop Codon: 1
Protein Length: 575 | Molecular Weight (kDa): 67.802
UniProt ID(s): Q8I2A1
PDB ID(s): None
Isoelectric Point: 9.47
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005052 | Legume-like lectin |
| InterPro | IPR013320 | Concanavalin A-like lectin/glucanase domain superfamily |
| PFam | PF03388 | Legume-like lectin |
| Superfamily | SSF49899 | Concanavalin A-like lectin/glucanase domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 33515807 | Systematic Identification of Plasmodium Falciparum Sporozoite Membrane ProteinInteractions Reveals an Essential Role for the p24 Complex in Host Infection. | Knockel J, Dundas K, ..., Wright GJ | 10.1074/mcp.RA120.002432 |