PF3D7_0104000 (TRSP)

Genome location: Pf3D7_01_v3:175,609..176,376(+)

Genome classification: Core

Function and Localization

Product Description: thrombospondin-related sporozoite protein

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0009986	cell surface
Process	GO:0044409	entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.00	1.00
Trophozoite	not expressed	0.02	0.99
Schizont	not expressed	0.02	0.99
Gametocyte	not expressed	0.02	0.99

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0104000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.989 | MFS: -0.714 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0209100)

Modification: Disrupted | RMgm-4545

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: M1EUE6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532694

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	6	3	8
disruptive	3	8	9	25
missense	3	8	9	21

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	14	4	15
disruptive	7	24	24	27
missense	5	18	22	21

PlasmoDB Total SNPs: 82

Non-coding: 75 | Synonymous: 4 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 163 | Molecular Weight (kDa): 19.334

UniProt ID(s): M1EUE6, Q8I2A0

PDB ID(s): 5UY2, 5V2B, 5VR5

Isoelectric Point: 9.68

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000884	Thrombospondin type-1 (TSP1) repeat
InterPro	IPR036383	Thrombospondin type-1 (TSP1) repeat superfamily
InterPro	IPR043973	CCN, TSP1 domain
PFam	PF19035	CCN, TSP1 domain
Superfamily	SSF82895	Thrombospondin type-1 (TSP1) repeat superfamily

Associated Publications

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
17418435	Depletion of the Plasmodium berghei thrombospondin-related sporozoite proteinreveals a role in host cell entry by sporozoites.	Labaied M, Camargo N, Kappe SH	10.1016/j.molbiopara.2007.03.001
27128092	Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics.	Swearingen KE, Lindner SE, ..., Sinnis P	10.1371/journal.ppat.1005606
33083027	Discovery of four new B-cell protective epitopes for malaria using Q betavirus-like particle as platform	Atcheson E, Cabral-Miranda G, Salman AM, Reyes-Sandoval A	10.1038/s41541-020-00242-y
30305687	TRSP is dispensable for the Plasmodium pre-erythrocytic phase	Costa DM, Sa M, ..., Tavares J	10.1038/s41598-018-33398-8