Product Description: ubiquitin carboxyl-terminal hydrolase 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.19.12 (Ubiquitinyl hydrolase 1)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Component | GO:0000502 | proteasome complex |
| Function | GO:0004197 | cysteine-type endopeptidase activity |
| Function | GO:0004843 | thiol-dependent deubiquitinase |
| Process | GO:0006897 | endocytosis |
| Process | GO:0016579 | protein deubiquitination |
| Process | GO:0009410 | response to xenobiotic stimulus |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 3.29 | 0.09 |
| Trophozoite | expressed | 1.64 | 0.28 |
| Schizont | expressed | 1.36 | 0.42 |
| Gametocyte | not expressed | 1.31 | 0.40 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0104300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.292 | MFS: -2.606 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0208800)
Modification: Mutated | RMgm-4829
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.19.12 | ubiquitinyl hydrolase 1 | KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O75604 | Ubiquitin carboxyl-terminal hydrolase 2 | Homo sapiens | OrthoDB | LCACNLCAELCAHALCAE... |
| P40818 | Ubiquitin carboxyl-terminal hydrolase 8 | Homo sapiens | OrthoDB | CHEMBL2398212CHEMBL5172138CHEMBL5196686CHEMBL5173239... |
| Q9P275 | Ubiquitin carboxyl-terminal hydrolase 36 | OrthoDB | CHEMBL5172138CHEMBL5173239CHEMBL5185273 | |
| O94782 | Ubiquitin carboxyl-terminal hydrolase 1 | OrthoDB | 9-(4-(3,5-dimethyl-1H-US10189841, Compound I-2US10399980, Compound I-3US10399980, Compound I-4... | |
| Q70CQ3 | Ubiquitin carboxyl-terminal hydrolase 30 | OrthoDB | US11066365, Example 1US11390584, Example 2US11390584, Example 3US11390584, Example 4... |
Protein Length: 3499 | Molecular Weight (kDa): 416.039
UniProt ID(s): A0A5J6C8G6, A0A5J6C8J3, A0A5J6C998, A0A5J6C9A7, A0A5J6CAN8, A0A5J6CB24, A0A5J6CB44, A0A5J6CBC4, A0A5J6CBG6, A0A5J6CBU6, A0A5J6CC28, A0A5J6CCN9, Q8I296
PDB ID(s): None
Isoelectric Point: 8.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR018200 | Ubiquitin specific protease, conserved site |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 47 | 194 | 113 | 191 |
| disruptive | 233 | 627 | 282 | 704 |
| missense | 141 | 470 | 237 | 546 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 89 | 352 | 123 | 191 |
| disruptive | 414 | 1187 | 475 | 1060 |
| missense | 217 | 891 | 355 | 608 |
PlasmoDB Total SNPs: 873
Non-coding: 255 | Synonymous: 313 | Nonsynonymous: 305 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 31896710 | A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malariaparasites. | Birnbaum J, Scharf S, ..., Spielmann T | 10.1126/science.aax4735 |
| 34090067 | Plasmodium falciparum resistance to ACTs: Emergence, mechanisms, and outlook | Siddiqui FA, Liang X, Cui L | 10.1016/j.ijpddr.2021.05.007 |
| 33095255 | Artemisinin susceptibility in the malaria parasite Plasmodium falciparum:propellers, adaptor proteins and the need for cellular healing | Sutherland CJ, Henrici RC, Artavanis-Tsakonas K | 10.1093/femsre/fuaa056 |
| 31636063 | Modification of pfap2mu and pfubp1 Markedly Reduces Ring-Stage Susceptibility of Plasmodium falciparum to Artemisinin In Vitro | Henrici RC, van Schalkwyk DA, Sutherland CJ | 10.1128/AAC.01542-19 |
| 38413566 | Deaggregation of mutant Plasmodium yoelii de-ubiquitinase UBP1 alters MDR1localization to confer multidrug resistance | Xu R, Lin L, ..., Li J | 10.1038/s41467-024-46006-3 |
| 34309894 | The newly discovered role of endocytosis in artemisinin resistance | Behrens HM, Schmidt S, Spielmann T | 10.1002/med.21848 |
| 33536101 | Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9and DRBD7 | Erben E, Leiss K, ..., Clayton C | 10.1017/S0031182021000123 |
| 36889372 | Transcription factor cellular promoter 2 is required for upstream binding protein1 -mediated angiogenesis | Ren Y, YaneYang, ..., Zhou J | 10.1016/j.gep.2023.119308 |
| 38389957 | RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression isaltered by CRISPR-Cas9 gene editing and overexpression | Sabalette KB, Campo VA, ..., De Gaudenzi JG | 10.1016/j.dib.2024.110156 |
| 33903109 | Characterization of pfmdr1, pfcrt, pfK13, pfubp1, and pfap2mu in TravelersReturning from Africa with Plasmodium falciparum Infections Reported in Chinafrom 2014 to 2018 | Feng J, Xu D, ..., Xia Z | 10.1128/AAC.02717-20 |
| 31713515 | Cycles of autoubiquitination and deubiquitination regulate the ERAD ubiquitinligase Hrd1 | Peterson BG, Glaser ML, Rapoport TA, Baldridge RD | 10.7554/eLife.50903 |
| 38348324 | Proteomic data of the Trypanosoma cruzi insect-dwelling epimastigotesoverexpressing the RNA-binding protein UBP1 | Sabalette KB, Campo VA, De Gaudenzi JG | 10.1016/j.dib.2024.110085 |
| 35294497 | Phosphorylated viral protein evades plant immunity through interfering thefunction of RNA-binding protein | Li J, Feng H, ..., Chen J | 10.1371/journal.ppat.1010412 |
| 32340987 | Experimentally Engineered Mutations in a Ubiquitin Hydrolase, UBP-1, Modulate InVivo Susceptibility to Artemisinin and Chloroquine in Plasmodium berghei | Simwela NV, Hughes KR, ..., Waters AP | 10.1128/AAC.02484-19 |