PF3D7_0104800 (NPT1)

Genome location: Pf3D7_01_v3:215,143..217,616(+)

Genome classification: Core

Function and Localization

Product Description: novel putative transporter 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 12

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0071944	cell periphery
Component	GO:0005886	plasma membrane
Process	GO:0015819	lysine transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.09	0.95
Trophozoite	expressed	0.25	0.84
Schizont	expressed	0.34	0.79
Gametocyte	possibly expressed	0.05	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0104800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.781 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0208300)

Relative Growth Rate: 0.95 ± 0.13
Confidence: 5.42

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PbANKA_0208300)

Modification: Tagged | RMgm-4330

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I291

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_129777

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	32	15	42
disruptive	7	39	23	58
missense	7	30	21	38

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	62	35	45
disruptive	19	99	58	189
missense	16	71	43	107

PlasmoDB Total SNPs: 91

Non-coding: 57 | Synonymous: 25 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 577 | Molecular Weight (kDa): 65.425

UniProt ID(s): Q8I291

PDB ID(s): None

Isoelectric Point: 8.57

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR036259	MFS transporter superfamily
PFam	N/A	N/A
Superfamily	SSF103473	MFS transporter superfamily

Associated Publications

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
21752110	The novel putative transporter NPT1 plays a critical role in early stages ofPlasmodium berghei sexual development.	Boisson B, Lacroix C, ..., Baldacci P	10.1111/j.1365-2958.2011.07767.x
28205520	Cationic amino acid transporters play key roles in the survival and transmissionof apicomplexan parasites.	Rajendran E, Hapuarachchi SV, ..., van Dooren GG	10.1038/ncomms14455
31915223	Influence of Plasmodium falciparum Calcium-Dependent Protein Kinase 5 (Pf CDPK5)on the Late Schizont Stage Phosphoproteome	Blomqvist K, Helmel M, ..., Dvorin JD	10.1128/mSphere.00921-19
34756057	Characterization of Apicomplexan Amino Acid Transporters (ApiATs) in the MalariaParasite Plasmodium falciparum.	Wichers JS, van Gelder C, ..., Bachmann A	10.1128/mSphere.00743-21
32807664	Mathematical modeling analysis of hepatic uric acid disposition using humansandwich-cultured hepatocytes	Wada S, Matsunaga N, Tamai I	10.1016/j.dmpk.2020.06.006
32121619	RHDeltagra17Deltanpt1 Strain of Toxoplasma gondii Elicits Protective Immunity AgainstAcute, Chronic and Congenital Toxoplasmosis in Mice	Liang QL, Sun LX, ..., Wang JL	10.3390/microorganisms8030352