PF3D7_0105200 (RAP1)

Genome location: Pf3D7_01_v3:222,086..224,501(-)

Genome classification: Core

Function and Localization

Product Description: RAP protein RAP1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0003729	mRNA binding
Function	GO:0097177	mitochondrial ribosome binding
Function	GO:0019843	rRNA binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.15	0.93
Trophozoite	expressed	0.79	0.58
Schizont	expressed	0.27	0.82
Gametocyte	expressed	0.61	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0105200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.8 | MFS: -2.879 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0208100)

Relative Growth Rate: 0.07 ± 0.08
Confidence: 6.37

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0208100)

Modification: Tagged | RMgm-4465

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I289

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_139431

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	21	16	30
disruptive	5	27	29	70
missense	5	27	29	63

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	54	27	33
disruptive	9	78	66	133
missense	8	63	59	108

PlasmoDB Total SNPs: 98

Non-coding: 74 | Synonymous: 17 | Nonsynonymous: 5 | Stop Codon: 2

Protein Information

Protein Length: 568 | Molecular Weight (kDa): 67.157

UniProt ID(s): Q8I289

PDB ID(s): None

Isoelectric Point: 8.21

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013584	RAP domain
PFam	PF08373	RAP domain
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
27381095	The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum.	Bunnik EM, Batugedara G, ..., Le Roch KG	10.1186/s13059-016-1014-0
30102371	Identification of clustered organellar short (cos) RNAs and of a conserved familyof organellar RNA-binding proteins, the heptatricopeptide repeat proteins, in themalaria parasite.	Hillebrand A, Matz JM, ..., Schmitz-Linneweber C	10.1093/nar/gky710
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21
35277503	Functional genomics of RAP proteins and their role in mitoribosome regulation inPlasmodium falciparum.	Hollin T, Abel S, ..., Le Roch KG	10.1038/s41467-022-28981-7
38387847	Comparative transcriptomics reveals common and strain-specific responses of humanmacrophages to infection with Mycobacterium tuberculosis and Mycobacterium bovisBCG	Li P, Li Y, Wang CC, Xia LG	10.1016/j.micpath.2024.106593
38320624	Rap1GAP exacerbates myocardial infarction by regulating the AMPK/SIRT1/NF-kappa B signaling pathway	Shan T, Li X, ..., Zhao Z	10.1016/j.cellsig.2024.111080
38342414	rpoS involved in immune response of Macrobrachium nipponens to Vibrio mimicus infection	Jiang Z, Qin L, ..., Zhang X	10.1016/j.fsi.2024.109440