PF3D7_0105600

Genome location: Pf3D7_01_v3:229,943..234,464(-)

Genome classification: Core

Function and Localization

Product Description: FtsJ-like methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0004483	mRNA (nucleoside-2'-O-)-methyltransferase activity
Process	GO:0006370	7-methylguanosine mRNA capping
Process	GO:0097309	cap1 mRNA methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.28	0.86
Trophozoite	expressed	0.56	0.68
Schizont	expressed	0.22	0.86
Gametocyte	expressed	0.56	0.69

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0105600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.042 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0207700)

Modification: Other | RMgm-451

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I285

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=6.34) with 6MT9 (Global RMSD=15.51)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105981

Most Similar Human Ortholog: Q8IYT2

TM-align score: 0.57 | RMSD: 5.57

Seq Identity: 0.18 | Length: 542 / 1224

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000180917	cap methyltransferase 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	50	29	102
disruptive	32	112	76	185
missense	26	107	74	170

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	116	49	101
disruptive	52	262	120	254
missense	39	233	112	204

PlasmoDB Total SNPs: 201

Non-coding: 124 | Synonymous: 46 | Nonsynonymous: 31 | Stop Codon: 0

Protein Information

Protein Length: 1224 | Molecular Weight (kDa): 147.395

UniProt ID(s): Q8I285

PDB ID(s): None

Isoelectric Point: 6.86

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002877	Ribosomal RNA methyltransferase FtsJ domain
PFam	PF01728	Ribosomal RNA methyltransferase FtsJ domain
Superfamily	N/A	N/A