PF3D7_0105900

Genome location: Pf3D7_01_v3:253,089..255,771(+)

Genome classification: Core

Function and Localization

Product Description: 3'-5' exonuclease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.11.- (Exodeoxyribonucleases producing 5'-phosphomonoesters.);3.1.11.1 (Exodeoxyribonuclease I)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0008408	3'-5' exonuclease activity
Function	GO:0003677	DNA binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.10	0.95
Trophozoite	expressed	0.78	0.58
Schizont	expressed	0.57	0.66
Gametocyte	expressed	0.84	0.56

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0105900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.886 | MFS: -2.507 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I282

"Best" AlphaFill ligand hit: DGP (2'-deoxyguanosine-5'-monophosphate, Local RMSD=1.10) with 2FC0 (Global RMSD=3.37)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.11.-	Ex: exodeoxyribonuclease V	NTPDNAPNAKClATPEDTAZnCl2sulfatephosphated(GCTGGTGG)d(GCAGGTGG)abc protein...
3.1.11.1	exodeoxyribonuclease I	NTPEDTAcamptothecinendonuclease CtIPfullerene-oligonucleotide conjugatesingle-stranded DNA-binding protein5'-thiophosphorylated oligonucleotide

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106571

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	14	16	39
disruptive	5	46	25	62
missense	5	45	23	55

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	41	25	38
disruptive	17	112	55	114
missense	9	98	45	71

PlasmoDB Total SNPs: 151

Non-coding: 128 | Synonymous: 16 | Nonsynonymous: 6 | Stop Codon: 1

Protein Information

Protein Length: 448 | Molecular Weight (kDa): 53.423

UniProt ID(s): Q8I282

PDB ID(s): None

Isoelectric Point: 8.94

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002562	3'-5' exonuclease domain
InterPro	IPR012337	Ribonuclease H-like superfamily
PFam	PF01612	3'-5' exonuclease domain
Superfamily	SSF53098	Ribonuclease H-like superfamily