PF3D7_0106400

Genome location: Pf3D7_01_v3:272,296..273,701(+)

Genome classification: Core

Function and Localization

Product Description: pre-rRNA-processing protein TSR2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0000462	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.81	0.64
Trophozoite	expressed	0.72	0.64
Schizont	expressed	0.12	0.92
Gametocyte	expressed	0.76	0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0106400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.154 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0206900)

Relative Growth Rate: 0.07 ± 0.07
Confidence: 6.80

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I278

"Best" AlphaFill ligand hit: GTP (guanosine-5'-triphosphate, Local RMSD=1.01) with 2R7X (Global RMSD=14.52)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103365

Most Similar Human Ortholog: Q969E8

TM-align score: 0.67 | RMSD: 1.56

Seq Identity: 0.20 | Length: 114 / 156

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000158526	TSR2 ribosome maturation factor

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	7	5	8
disruptive	1	16	9	25
missense	1	15	9	22

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	11	5	10
disruptive	2	39	20	53
missense	2	35	18	33

PlasmoDB Total SNPs: 142

Non-coding: 131 | Synonymous: 4 | Nonsynonymous: 6 | Stop Codon: 1

Protein Information

Protein Length: 156 | Molecular Weight (kDa): 18.395

UniProt ID(s): Q8I278

PDB ID(s): None

Isoelectric Point: 4.1

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR019398	Pre-rRNA-processing protein TSR2
PFam	PF10273	Pre-rRNA-processing protein TSR2
Superfamily	N/A	N/A