Product Description: ras-related protein Rab-5C
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.2 (Small monomeric GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0030139 | endocytic vesicle |
| Component | GO:0012505 | endomembrane system |
| Component | GO:0005768 | endosome |
| Function | GO:0003924 | GTPase activity |
| Process | GO:0006886 | intracellular protein transport |
| Process | GO:0007264 | small GTPase mediated signal transduction |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.48 | 0.71 |
| Schizont | expressed | 0.34 | 0.78 |
| Gametocyte | possibly expressed | 0.36 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0106800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.264 | MFS: -2.504 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0206500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0206500)
Modification: Disrupted | RMgm-1688
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I274
"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.12) with 1Z0D (Global RMSD=1.25)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.2 | small monomeric GTPase | GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100743
Most Similar Human Ortholog: F8VUA5
TM-align score: 0.87 | RMSD: 1.52
Seq Identity: 0.62 | Length: 106 / 214
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000108774 | RAB5C, member RAS oncogene family |
| ENSG00000111540 | RAB5B, member RAS oncogene family |
| ENSG00000144566 | RAB5A, member RAS oncogene family |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 7 | 7 | 11 |
| disruptive | 1 | 7 | 5 | 15 |
| missense | 1 | 7 | 4 | 12 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 21 | 6 | 19 |
| disruptive | 3 | 24 | 19 | 64 |
| missense | 1 | 19 | 10 | 47 |
PlasmoDB Total SNPs: 141
Non-coding: 133 | Synonymous: 6 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 214 | Molecular Weight (kDa): 24.301
PDB ID(s): None
Isoelectric Point: 7.24
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001806 | Small GTPase |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00071 | Small GTPase |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23223036 | Isoprenoid biosynthesis inhibition disrupts Rab5 localization and food vacuolarintegrity in Plasmodium falciparum. | Howe R, Kelly M, ..., Odom AR | 10.1128/EC.00073-12 |