Product Description: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.60 (2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0050518 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity |
| Process | GO:0035690 | cellular response to drug |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.06 | 0.97 |
| Trophozoite | possibly expressed | 0.66 | 0.64 |
| Schizont | expressed | 0.43 | 0.75 |
| Gametocyte | expressed | 0.69 | 0.63 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0106900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.124 | MFS: -2.887 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0206400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I273
"Best" AlphaFill ligand hit: C5P (cytidine-5'-monophosphate, Local RMSD=11.23) with 1W77 (Global RMSD=15.90)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.60 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase | CTPcefepimequercetinMMV008138rifampicintunicamycindequaliniumfosmidomycinrosuvastatinsulfanilamidechelerythrineketoclomazone... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P69834 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic | OrthoMCL, OrthoMCL BLAST | CHEMBL2289493CHEMBL2289491CHEMBL2289489CHEMBL2289492 |
Ortholog Group (OrthoMCL): OG6_103820
Most Similar Human Ortholog: A0A6Q8PHI3
TM-align score: 0.54 | RMSD: 3.24
Seq Identity: 0.17 | Length: 187 / 734
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000214960 | CDP-L-ribitol pyrophosphorylase A |
Protein Length: 734 | Molecular Weight (kDa): 88.194
UniProt ID(s): A0A219YKQ2, A0A219YKQ3, Q8I273
PDB ID(s): None
Isoelectric Point: 9.89
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR018294 | 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, conserved site |
| InterPro | IPR029044 | Nucleotide-diphospho-sugar transferases |
| InterPro | IPR034683 | Cytidylyltransferase IspD/TarI |
| PFam | PF01128 | Cytidylyltransferase IspD/TarI |
| Superfamily | SSF53448 | Nucleotide-diphospho-sugar transferases |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 28 | 24 | 48 |
| disruptive | 28 | 100 | 69 | 136 |
| missense | 21 | 79 | 57 | 115 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 75 | 37 | 54 |
| disruptive | 62 | 239 | 119 | 261 |
| missense | 36 | 174 | 93 | 170 |
PlasmoDB Total SNPs: 151
Non-coding: 65 | Synonymous: 55 | Nonsynonymous: 31 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25367906 | A chemical rescue screen identifies a Plasmodium falciparum apicoplast inhibitortargeting MEP isoprenoid precursor biosynthesis. | Wu W, Herrera Z, ..., Yeh E | 10.1128/AAC.03342-14 |
| 29958953 | Deciphering the role of IspD (2_C_methyl_D_erythritol 4_phosphatecytidyltransferase) enzyme as a potential therapeutic drug target againstPlasmodium vivax. | Saggu GS, Garg S, ..., Saxena V | 10.1016/j.gene.2018.06.084 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 38305785 | Crystal structure and biophysical characterization of IspD from Burkholderiathailandensis and Mycobacterium paratuberculosis | Pierce PG, Hartnett BE, ..., Myler PJ | 10.1107/S2053230X24000621 |