Product Description: eukaryotic translation initiation factor 2-alpha kinase 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0022626 | cytosolic ribosome |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004694 | eukaryotic translation initiation factor 2alpha kinase activity |
| Function | GO:0004672 | protein kinase activity |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0010998 | regulation of translational initiation by eIF2 alpha phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.19 | 0.91 |
| Trophozoite | not expressed | 0.13 | 0.93 |
| Schizont | not expressed | 0.08 | 0.96 |
| Gametocyte | possibly expressed | 0.88 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0107600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.835 | #Insertions: 2
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0205800)
Modification: Disrupted | RMgm-1682
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I265
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.50) with 6D3K (Global RMSD=3.94)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 72 | 36 | 96 |
| disruptive | 71 | 232 | 136 | 286 |
| missense | 44 | 188 | 125 | 229 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 156 | 64 | 121 |
| disruptive | 144 | 572 | 221 | 509 |
| missense | 78 | 422 | 173 | 312 |
PlasmoDB Total SNPs: 317
Non-coding: 72 | Synonymous: 145 | Nonsynonymous: 99 | Stop Codon: 1
Protein Length: 1595 | Molecular Weight (kDa): 194.631
UniProt ID(s): Q8I265
PDB ID(s): None
Isoelectric Point: 10.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 20584882 | The Plasmodium eukaryotic initiation factor-2alpha kinase IK2 controls thelatency of sporozoites in the mosquito salivary glands. | Zhang M, Fennell C, ..., Nussenzweig V | 10.1084/jem.20091975 |
| 36810637 | A non-canonical sensing pathway mediates Plasmodium adaptation to amino aciddeficiency | Marreiros IM, Marques S, ..., Mota MM | 10.1038/s42003-023-04566-y |
| 28256794 | Laser capture microdissection enables transcriptomic analysis of dividing andquiescent liver stages of Plasmodium relapsing species | Cubi R, Vembar SS, ..., Mazier D | 10.1111/cmi.12735 |