Product Description: double-strand break repair protein MRE11
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.11.- (Exodeoxyribonucleases producing 5'-phosphomonoesters.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0030870 | Mre11 complex |
| Component | GO:0005634 | nucleus |
| Component | GO:0035861 | site of double-strand break |
| Function | GO:0004518 | nuclease activity |
| Function | GO:0005515 | protein binding |
| Function | GO:0000014 | single-stranded DNA endodeoxyribonuclease activity |
| Process | GO:0006281 | DNA repair |
| Process | GO:0000724 | double-strand break repair via homologous recombination |
| Process | GO:0006303 | double-strand break repair via nonhomologous end joining |
| Process | GO:0042138 | meiotic DNA double-strand break formation |
| Process | GO:0097552 | mitochondrial double-strand break repair via homologous recombination |
| Process | GO:0007095 | mitotic G2 DNA damage checkpoint signaling |
| Process | GO:0000723 | telomere maintenance |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.15 | 0.93 |
| Trophozoite | expressed | 0.86 | 0.54 |
| Schizont | expressed | 0.23 | 0.86 |
| Gametocyte | expressed | 0.41 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0107800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.289 | #Insertions: 6
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0205600)
Modification: Tagged | RMgm-4877
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZUM0
"Best" AlphaFill ligand hit: DW0 (1,1'-propane-1,3-diylbis(3,7-dimethyl-3,7-dihydro-1h-purine-2,6-dione), Local RMSD=0.19) with 8OSE (Global RMSD=15.31)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.11.- | Ex: exodeoxyribonuclease V | NTPDNAPNAKClATPEDTAZnCl2sulfatephosphated(GCTGGTGG)d(GCAGGTGG)abc protein... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P49959 | Double-strand break repair protein MRE11 | OrthoDB | US11453663, Preparative Example 129US11453663, Preparative Example 130US11453663, Preparative Example 131US11453663, Preparative Example 132... |
Ortholog Group (OrthoMCL): OG6_101854
Most Similar Human Ortholog: F5GXT0
TM-align score: 0.86 | RMSD: 2.33
Seq Identity: 0.38 | Length: 203 / 1233
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000020922 | MRE11 homolog, double strand break repair nuclease |
Protein Length: 1233 | Molecular Weight (kDa): 146.148
UniProt ID(s): A0A143ZUM0
PDB ID(s): None
Isoelectric Point: 5.35
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007281 | Mre11, DNA-binding |
| PFam | PF04152 | Mre11, DNA-binding |
| Superfamily | SSF56300 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 56 | 35 | 85 |
| disruptive | 69 | 199 | 90 | 233 |
| missense | 36 | 151 | 72 | 175 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 27 | 129 | 49 | 87 |
| disruptive | 130 | 391 | 164 | 357 |
| missense | 75 | 288 | 123 | 221 |
PlasmoDB Total SNPs: 186
Non-coding: 36 | Synonymous: 73 | Nonsynonymous: 77 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25938776 | Identification of Plasmodium falciparum DNA Repair Protein Mre11 with anEvolutionarily Conserved Nuclease Function. | Badugu SB, Nabi SA, ..., Bhattacharyya MK | 10.1371/journal.pone.0125358 |
| 33239368 | Elucidation of D NA Repair Function of Pf Blm and Potentiation of Artemisinin Action by a Small-Molecule Inhibitor of Rec Q Helicase | Suthram N, Padhi S, ..., Bhattacharyya MK | 10.1128/mSphere.00956-20 |
| 33287434 | M RE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life | Guttery DS, Ramaprasad A, ..., Tewari R | 10.3390/cells9122590 |
| 38217957 | Xrs2/NBS1 promote end-bridging activity of the MRE11-RAD50 complex | Moller C, Sharma R, ..., Westerlund F | 10.1016/j.bbrc.2023.149464 |