PF3D7_0108000

Genome location: Pf3D7_01_v3:328,171..329,995(-)

Genome classification: Core

Function and Localization

Product Description: proteasome subunit beta type-3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Component	GO:0005839	proteasome core complex
Component	GO:0019774	proteasome core complex, beta-subunit complex
Function	GO:0004175	endopeptidase activity
Process	GO:0010498	proteasomal protein catabolic process
Process	GO:0010499	proteasomal ubiquitin-independent protein catabolic process
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.10	0.95
Trophozoite	expressed	0.42	0.74
Schizont	expressed	0.21	0.86
Gametocyte	expressed	0.20	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0108000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -3.212 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I261

"Best" AlphaFill ligand hit: 79P ((2~{s})-3-(1~{h}-indol-3-yl)-~{n}-[(2~{s},3~{s},4~{r})-4-methyl-3,5-bis(oxidanyl)-1-phenyl-pentan-2-yl]-2-[[(2~{r})-2-(2-morpholin-4-ylethanoylamino)propanoyl]amino]propanamide, Local RMSD=0.07) with 5L5O (Global RMSD=2.24)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.25.1	proteasome endopeptidase complex	ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101970

Most Similar Human Ortholog: P49720

TM-align score: 0.98 | RMSD: 0.79

Seq Identity: 0.46 | Length: 205 / 218

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000275903	proteasome 20S subunit beta 3
ENSG00000277791	proteasome 20S subunit beta 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	9	9	10
disruptive	1	7	3	22
missense	0	5	2	17

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	20	9	12
disruptive	2	17	14	60
missense	0	12	12	46

PlasmoDB Total SNPs: 138

Non-coding: 133 | Synonymous: 4 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 218 | Molecular Weight (kDa): 24.51

UniProt ID(s): Q8I261

PDB ID(s): 5FMG

Isoelectric Point: 4.78

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001353	Proteasome, subunit alpha/beta
InterPro	IPR016050	Proteasome beta-type subunit, conserved site
InterPro	IPR029055	Nucleophile aminohydrolases, N-terminal
InterPro	IPR033811	Proteasome beta 3 subunit
PFam	PF00227	Proteasome, subunit alpha/beta
Superfamily	SSF56235	Nucleophile aminohydrolases, N-terminal