PF3D7_0109000 (PHIL1)

Genome location: Pf3D7_01_v3:362,217..365,209(+)

Genome classification: Core

Function and Localization

Product Description: photosensitized INA-labeled protein PHIL1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0070258	inner membrane pellicle complex
Function	GO:0005515	protein binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.42	0.85
Trophozoite	not expressed	0.03	0.99
Schizont	expressed	0.61	0.73
Gametocyte	expressed	0.50	0.72

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0109000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.122 | MFS: -3.121 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0204600)

Relative Growth Rate: 0.30 ± 0.48
Confidence: 2.86

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0204600)

Modification: Tagged | RMgm-5129

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I253

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_215312

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	3	5	12
disruptive	0	7	6	17
missense	0	7	6	13

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	10	13	12
disruptive	2	20	21	43
missense	2	16	16	38

PlasmoDB Total SNPs: 251

Non-coding: 247 | Synonymous: 1 | Nonsynonymous: 2 | Stop Codon: 1

Protein Information

Protein Length: 224 | Molecular Weight (kDa): 25.477

UniProt ID(s): Q8I253

PDB ID(s): None

Isoelectric Point: 4.55

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
28985225	Disrupting assembly of the inner membrane complex blocks Plasmodium falciparumsexual stage development.	Parkyn Schneider M, Liu B, ..., Dixon MWA	10.1371/journal.ppat.1006659
29138437	Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the innermembrane complex and is required for Plasmodium parasite development.	Saini E, Zeeshan M, ..., Tewari R	10.1038/s41598-017-15781-z
34324609	Plasmodium falciparum PhIL1-associated complex plays an essential role inmerozoite reorientation and invasion of host erythrocytes.	Saini E, Sheokand PK, ..., Malhotra P	10.1371/journal.ppat.1009750
26468752	Global Analysis of Palmitoylated Proteins in Toxoplasma gondii	Foe IT, Child MA, ..., Bogyo M	10.1016/j.chom.2015.09.006
17030994	Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondiipellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide	Gilk SD, Raviv Y, ..., Ward GE	10.1128/EC.00114-06
33830607	Identification of novel inner membrane complex and apical annuli proteins of themalaria parasite Plasmodium falciparum	Wichers JS, Wunderlich J, ..., Gilberger TW	10.1111/cmi.13341