PF3D7_0109400

Genome location: Pf3D7_01_v3:373,864..375,683(-)

Genome classification: Core

Function and Localization

Product Description: tubulin-specific chaperone a, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0015630	microtubule cytoskeleton
Function	GO:0015631	tubulin binding
Function	GO:0051082	unfolded protein binding
Process	GO:0006457	protein folding
Process	GO:0065003	protein-containing complex assembly
Process	GO:0007021	tubulin complex assembly

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.11	0.95
Trophozoite	expressed	0.35	0.80
Schizont	expressed	0.10	0.94
Gametocyte	expressed	1.78	0.26

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0109400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.24 | MFS: -3.364 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0204200)

Relative Growth Rate: 0.96 ± 0.12
Confidence: 5.56

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0204200)

Modification: Disrupted | RMgm-1678

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I250

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=9.91) with 3PLA (Global RMSD=13.38)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102635

Most Similar Human Ortholog: O75347

TM-align score: 0.86 | RMSD: 1.97

Seq Identity: 0.25 | Length: 108 / 166

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000171530	tubulin folding cofactor A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 127

Non-coding: 119 | Synonymous: 4 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 166 | Molecular Weight (kDa): 19.591

UniProt ID(s): Q8I250

PDB ID(s): None

Isoelectric Point: 5.23

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004226	Tubulin binding cofactor A
InterPro	IPR036126	Tubulin binding cofactor A superfamily
PFam	PF02970	Tubulin binding cofactor A
Superfamily	SSF46988	Tubulin binding cofactor A superfamily