PF3D7_0109500

Genome location: Pf3D7_01_v3:376,164..377,704(-)

Genome classification: Core

Function and Localization

Product Description: N-acetyltransferase, GNAT family, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.1 (Amino-acid N-acetyltransferase);2.3.1.48 (Histone acetyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0031416	NatB complex
Function	GO:0008080	N-acetyltransferase activity
Function	GO:0004596	peptide alpha-N-acetyltransferase activity
Process	GO:0017196	N-terminal peptidyl-methionine acetylation
Process	GO:0006474	N-terminal protein amino acid acetylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.12	0.94
Trophozoite	not expressed	0.21	0.87
Schizont	not expressed	0.04	0.97
Gametocyte	expressed	0.30	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0109500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.659 | MFS: -2.369 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I249

"Best" AlphaFill ligand hit: CMC (carboxymethyl coenzyme *a, Local RMSD=1.00) with 6ZMP (Global RMSD=2.58)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.1	amino-acid N-acetyltransferase	CoAKClArgEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4FeCl3BaCl2...
2.3.1.48	histone acetyltransferase	CoADNARNABF1EDTAMB-3LTK14PU139A-485TTK21CPTH2CPTH6...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102129

Most Similar Human Ortholog: P61599

TM-align score: 0.95 | RMSD: 1.03

Seq Identity: 0.45 | Length: 152 / 155

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000173418	N-alpha-acetyltransferase 20, NatB catalytic subunit

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	1
disruptive	0	0	0	0
missense	0	0	0	0

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	1
disruptive	0	0	0	0
missense	0	0	0	0

PlasmoDB Total SNPs: 64

Non-coding: 63 | Synonymous: 1 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 155 | Molecular Weight (kDa): 18.23

UniProt ID(s): Q8I249

PDB ID(s): None

Isoelectric Point: 5.23

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013653	FR47-like
InterPro	IPR016181	Acyl-CoA N-acyltransferase
PFam	PF08445	FR47-like
Superfamily	SSF55729	Acyl-CoA N-acyltransferase