PF3D7_0110100 (SELB)

Genome location: Pf3D7_01_v3:388,887..392,185(-)

Genome classification: Core

Function and Localization

Product Description: selenocysteine-specific elongation factor, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003746	translation elongation factor activity
Process	GO:0001514	selenocysteine incorporation
Process	GO:0006414	translational elongation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.11	0.95
Trophozoite	possibly expressed	0.28	0.83
Schizont	possibly expressed	0.06	0.96
Gametocyte	expressed	0.33	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0110100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.697 | #Insertions: 4

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0203500)

Relative Growth Rate: 0.89 ± 0.18
Confidence: 4.79

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0203500)

Modification: Disrupted | RMgm-1675

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I243

"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.28) with 3WNB (Global RMSD=6.35)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103847

Most Similar Human Ortholog: C9J8T0

TM-align score: 0.82 | RMSD: 3.55

Seq Identity: 0.29 | Length: 403 / 934

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000132394	eukaryotic elongation factor, selenocysteine-tRNA specific
ENSG00000284869	eukaryotic elongation factor, selenocysteine-tRNA specific

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	41	17	33
disruptive	65	96	40	98
missense	40	64	34	77

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	16	60	35	34
disruptive	92	171	66	161
missense	51	117	49	113

PlasmoDB Total SNPs: 249

Non-coding: 4 | Synonymous: 116 | Nonsynonymous: 129 | Stop Codon: 0

Protein Information

Protein Length: 934 | Molecular Weight (kDa): 108.585

UniProt ID(s): Q8I243

PDB ID(s): None

Isoelectric Point: 7.28

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000795	Transcription factor, GTP-binding domain
InterPro	IPR009000	Translation protein, beta-barrel domain superfamily
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00009	Transcription factor, GTP-binding domain
Superfamily	SSF50447	Translation protein, beta-barrel domain superfamily
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase