Product Description: DNA-directed RNA polymerase II subunit RPB9, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
| Process | GO:0001193 | maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter |
| Process | GO:0006366 | transcription by RNA polymerase II |
| Process | GO:0006367 | transcription initiation from RNA polymerase II promoter |
| Process | GO:0006283 | transcription-coupled nucleotide-excision repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.29 | 0.85 |
| Trophozoite | expressed | 0.66 | 0.62 |
| Schizont | expressed | 0.15 | 0.90 |
| Gametocyte | expressed | 0.71 | 0.61 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0110400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.486 | MFS: -2.733 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0203300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0203300)
Modification: Disrupted | RMgm-1673
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I241
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102455
Most Similar Human Ortholog: P36954
TM-align score: 0.73 | RMSD: 2.32
Seq Identity: 0.37 | Length: 113 / 249
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000105258 | RNA polymerase II subunit I |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 13 | 8 | 15 |
| disruptive | 6 | 20 | 7 | 22 |
| missense | 2 | 16 | 6 | 16 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 30 | 14 | 6 |
| disruptive | 10 | 40 | 23 | 58 |
| missense | 4 | 29 | 17 | 37 |
PlasmoDB Total SNPs: 84
Non-coding: 75 | Synonymous: 5 | Nonsynonymous: 2 | Stop Codon: 2
Protein Length: 249 | Molecular Weight (kDa): 29.688
UniProt ID(s): Q8I241
PDB ID(s): None
Isoelectric Point: 4.41
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001529 | DNA-directed RNA polymerase, M/15kDa subunit |
| InterPro | IPR019761 | DNA-directed RNA polymerase M, 15kDa subunit, conserved site |
| InterPro | IPR034012 | Pol II subunit B9, C-terminal zinc ribbon |
| PFam | PF02150 | DNA-directed RNA polymerase, M/15kDa subunit |
| Superfamily | SSF57783 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 26429063 | Transcription coupled nucleotide excision repair in the yeast Saccharomycescerevisiae: The ambiguous role of Rad26 | Li S | 10.1016/j.dnarep.2015.09.006 |
| 35618649 | Absence of the Rpb9 subunit of RNA polymerase II reduces the chronological lifespan in fission yeast | Bhardwaj V, Sharma N | 10.1002/jobm.202200036 |
| 27796045 | Facilitators and Repressors of Transcription-coupled DNA Repair in Saccharomycescerevisiae | Li W, Li S | 10.1111/php.12655 |
| 33775921 | Deletion of the non-essential Rpb9 subunit of RNA polymerase II results inpleiotropic phenotypes in Schizosaccharomyces pombe | Bhardwaj V, Vishwakarma P, Lynn A, Sharma N | 10.1016/j.bbapap.2021.140654 |
| 32460824 | Taf14 is required for the stabilization of transcription pre-initiation complexin Saccharomyces cerevisiae | Peil K, Jurgens H, ..., Kristjuhan A | 10.1186/s13072-020-00347-7 |