Product Description: phosphatidylinositol-4-phosphate 5-kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.68 (1-phosphatidylinositol-4-phosphate 5-kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0016308 | 1-phosphatidylinositol-4-phosphate 5-kinase activity |
| Function | GO:0031267 | small GTPase binding |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.78 | 0.31 |
| Trophozoite | expressed | 1.29 | 0.38 |
| Schizont | expressed | 0.86 | 0.56 |
| Gametocyte | expressed | 1.23 | 0.40 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0110600
Resistome Missense Mutations: G60R
Resistome Compounds with Missense Mutations: MMV665928
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.2 | MFS: -2.765 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0203100)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0203100)
Modification: Tagged | RMgm-998
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I239
"Best" AlphaFill ligand hit: 5GP (guanosine-5'-monophosphate, Local RMSD=0.17) with 3X06 (Global RMSD=7.91)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.68 | 1-phosphatidylinositol-4-phosphate 5-kinase | ATPADPNEMEDTAPCMBGSSGZnSO4Cutsumheparincysteinesperminequercetin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9Y2I7 | 1-phosphatidylinositol 3-phosphate 5-kinase | OrthoDB | CHEMBL4446338CHEMBL2334586APILIMODCHEMBL2178735... | |
| O14986 | Phosphatidylinositol 4-phosphate 5-kinase type-1 beta | OrthoDB | CHEMBL203535CHEMBL5171272CHEMBL5206569CHEMBL5191668 | |
| Q99755 | Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha | Homo sapiens | OrthoDB | CHEMBL203535CHEMBL5171272CHEMBL5206569CHEMBL5191668 |
| O60331 | Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma | Homo sapiens | OrthoDB | CHEMBL1645107CHEMBL4640712CHEMBL4303547CHEMBL203535... |
| P48426 | Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha | OrthoDB | CHEMBL4072637CHEMBL4284040CHEMBL4550598CHEMBL4571099... | |
| P78356 | Phosphatidylinositol 5-phosphate 4-kinase type-2 beta | Homo sapiens | OrthoDB | ImanixilCHEMBL4533123CHEMBL4439287CHEMBL4446338... |
| Q8TBX8 | Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma | Homo sapiens | OrthoDB | US9623028, Compound 38CHEMBL2205766CHEMBL1529478CHEMBL5083265... |
Ortholog Group (OrthoMCL): OG6_100438
Most Similar Human Ortholog: A0A0A0MSP5
TM-align score: 0.75 | RMSD: 2.96
Seq Identity: 0.29 | Length: 141 / 1710
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000107242 | phosphatidylinositol-4-phosphate 5-kinase type 1 beta |
| ENSG00000143398 | phosphatidylinositol-4-phosphate 5-kinase type 1 alpha |
| ENSG00000186111 | phosphatidylinositol-4-phosphate 5-kinase type 1 gamma |
Protein Length: 1710 | Molecular Weight (kDa): 200.046
UniProt ID(s): Q8I239
PDB ID(s): None
Isoelectric Point: 7.94
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002498 | Phosphatidylinositol-4-phosphate 5-kinase, core |
| InterPro | IPR011992 | EF-hand domain pair |
| InterPro | IPR018247 | EF-Hand 1, calcium-binding site |
| InterPro | IPR023610 | Phosphatidylinositol-4-phosphate 5-kinase |
| PFam | PF01504 | Phosphatidylinositol-4-phosphate 5-kinase, core |
| Superfamily | SSF47473 | EF-hand domain pair |
| Superfamily | SSF56104 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 33 | 84 | 48 | 105 |
| disruptive | 150 | 228 | 95 | 257 |
| missense | 66 | 158 | 66 | 191 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 48 | 176 | 67 | 94 |
| disruptive | 218 | 459 | 217 | 477 |
| missense | 95 | 307 | 158 | 258 |
PlasmoDB Total SNPs: 566
Non-coding: 312 | Synonymous: 115 | Nonsynonymous: 139 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 19017115 | Drought stress and preharvest aflatoxin contamination in agricultural commodity:genetics, genomics and proteomics. | Guo B, Chen ZY, Lee RD, Scully BT | 10.1111/j.1744-7909.2008.00739.x |
| 19171150 | A unique phosphatidylinositol 4-phosphate 5-kinase is activated byADP-ribosylation factor in Plasmodium falciparum. | Leber W, Skippen A, ..., Baker DA | 10.1016/j.ijpara.2008.11.015 |
| 29326268 | Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics. | Cowell AN, Istvan ES, ..., Winzeler EA | 10.1126/science.aan4472 |
| 37834234 | PIP5Kgamma Mediates PI(4,5)P2/Merlin/LATS1 Signaling Activation and Interplays withHsc70 in Hippo-YAP Pathway Regulation | Le DDT, Le TPH, Lee SY | 10.3390/ijms241914786 |