PF3D7_0111300

Genome location: Pf3D7_01_v3:431,167..436,457(-)

Genome classification: Core

Function and Localization

Product Description: P-loop containing nucleoside triphosphate hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.1.15 (Nucleoside-triphosphate phosphatase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005663	DNA replication factor C complex
Component	GO:0005634	nucleus
Function	GO:0003677	DNA binding
Process	GO:0006260	DNA replication

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.08	0.96
Trophozoite	expressed	0.71	0.61
Schizont	expressed	0.34	0.80
Gametocyte	expressed	0.77	0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0111300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.812 | MFS: -2.874 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0202500)

Relative Growth Rate: 0.09 ± 0.09
Confidence: 6.29

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I233

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.1.15	nucleoside-triphosphate phosphatase	ATPADPNaFRNAEDTAdATPdTTPdCTPdGTPHOClrNTPMgCl2...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102931

Most Similar Human Ortholog: H3BT35

TM-align score: 0.34 | RMSD: 4.77

Seq Identity: 0.02 | Length: 64 / 1167

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000127586	chromosome transmission fidelity factor 18

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	49	35	55
disruptive	36	104	52	159
missense	20	84	42	135

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	89	42	61
disruptive	64	239	106	241
missense	35	171	82	185

PlasmoDB Total SNPs: 344

Non-coding: 240 | Synonymous: 69 | Nonsynonymous: 35 | Stop Codon: 0

Protein Information

Protein Length: 1167 | Molecular Weight (kDa): 139.214

UniProt ID(s): Q8I233

PDB ID(s): None

Isoelectric Point: 9.13

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003593	AAA+ ATPase domain
InterPro	IPR003959	ATPase, AAA-type, core
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00004	ATPase, AAA-type, core
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase