Product Description: TatD-like deoxyribonuclease
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.21.- (Endodeoxyribonucleases producing 5'-phosphomonoesters.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0009986 | cell surface |
| Component | GO:0033644 | host cell membrane |
| Component | GO:0005634 | nucleus |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0008296 | 3'-5'-exodeoxyribonuclease activity |
| Function | GO:0004536 | deoxyribonuclease activity |
| Process | GO:0006259 | DNA metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.18 | 0.91 |
| Trophozoite | possibly expressed | 0.42 | 0.75 |
| Schizont | expressed | 0.10 | 0.93 |
| Gametocyte | not expressed | 0.23 | 0.87 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0112000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -3.108 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0201800)
Modification: Mutated | RMgm-1447
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I226
"Best" AlphaFill ligand hit: 3D1 ((2r,3s,5r)-5-(6-amino-9h-purin-9-yl)-tetrahydro-2-(hydroxymethyl)furan-3-ol, Local RMSD=0.05) with 8EFG (Global RMSD=3.04)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.21.- | Ex: CC-preferring endodeoxyribonuclease | KClSDSDTTRNADNAATPEDTAEGTAtRNAactinNH4ClK3PO4... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100330
Most Similar Human Ortholog: E5RGF2
TM-align score: 0.95 | RMSD: 1.41
Seq Identity: 0.40 | Length: 210 / 447
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000147687 | TatD DNase domain containing 1 |
| ENSG00000203705 | TatD DNase domain containing 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 5 | 6 | 5 |
| disruptive | 1 | 4 | 13 | 31 |
| missense | 1 | 4 | 13 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 15 | 9 | 13 |
| disruptive | 4 | 34 | 38 | 84 |
| missense | 1 | 22 | 34 | 47 |
PlasmoDB Total SNPs: 55
Non-coding: 47 | Synonymous: 4 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 447 | Molecular Weight (kDa): 52.971
UniProt ID(s): Q8I226
PDB ID(s): None
Isoelectric Point: 9.02
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001130 | TatD family |
| InterPro | IPR018228 | Deoxyribonuclease, TatD-related, conserved site |
| InterPro | IPR032466 | Metal-dependent hydrolase |
| PFam | PF01026 | TatD family |
| Superfamily | SSF51556 | Metal-dependent hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 27151551 | The TatD-like DNase of Plasmodium is a virulence factor and a potential malariavaccine candidate. | Chang Z, Jiang N, ..., Chen Q | 10.1038/ncomms11537 |
| 29867907 | Plasmodium TatD-Like DNase Antibodies Blocked Parasite Development in theMosquito Gut. | Wang W, Liu F, ..., Chen Q | 10.3389/fmicb.2018.01023 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 34867886 | TatD DNases Contribute to Biofilm Formation and Virulence in Trueperellapyogenes | Zhang Z, Liang Y, ..., Liu M | 10.3389/fmicb.2021.758465 |
| 33420923 | TatD DNases of African trypanosomes confer resistance to host neutrophilextracellular traps | Zhang K, Jiang N, ..., Chen Q | 10.1007/s11427-020-1854-2 |
| 32431834 | A structural study of TatD from Staphylococcus aureus elucidates a putativeDNA-binding mode of a Mg(2+)-dependent nuclease | Lee KY, Cheon SH, ..., Lee BJ | 10.1107/S2052252520003917 |
| 36881763 | Human and bacterial TatD enzymes exhibit apurinic/apyrimidinic (AP) endonucleaseactivity | Dorival J, Eichman BF | 10.1093/nar/gkad133 |
| 30719750 | Computational and mutational analysis of TatD DNase of Bacillus anthracis | Singh D, Rahi A, ..., Bhatnagar R | 10.1002/jcb.28408 |