Product Description: heat shock protein 40, type II
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005829 | cytosol |
| Component | GO:0044164 | host cell cytosol |
| Function | GO:0051087 | chaperone binding |
| Function | GO:0015485 | cholesterol binding |
| Function | GO:0042393 | histone binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0051085 | chaperone cofactor-dependent protein refolding |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0009408 | response to heat |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.37 | 0.82 |
| Trophozoite | possibly expressed | 0.18 | 0.90 |
| Schizont | possibly expressed | 0.09 | 0.94 |
| Gametocyte | not expressed | 0.07 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0113700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.674 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0310000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2E1
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P31689 | DnaJ homolog subfamily A member 1 | Homo sapiens | OrthoDB | CHEMBL52073CHEMBL159171 |
Ortholog Group (OrthoMCL): OG6_100302
Most Similar Human Ortholog: H0Y4S1
TM-align score: 0.85 | RMSD: 1.51
Seq Identity: 0.46 | Length: 33 / 402
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132002 | DnaJ heat shock protein family (Hsp40) member B1 |
| ENSG00000137094 | DnaJ heat shock protein family (Hsp40) member B5 |
| ENSG00000162616 | DnaJ heat shock protein family (Hsp40) member B4 |
| ENSG00000187726 | DnaJ heat shock protein family (Hsp40) member B13 |
Protein Length: 402 | Molecular Weight (kDa): 46.988
UniProt ID(s): Q8I2E1
PDB ID(s): 6RZY
Isoelectric Point: 8.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001623 | DnaJ domain |
| InterPro | IPR002939 | Chaperone DnaJ, C-terminal |
| InterPro | IPR008971 | HSP40/DnaJ peptide-binding |
| InterPro | IPR018253 | DnaJ domain, conserved site |
| InterPro | IPR036869 | Chaperone J-domain superfamily |
| PFam | PF00226 | DnaJ domain |
| PFam | PF01556 | Chaperone DnaJ, C-terminal |
| Superfamily | SSF46565 | Chaperone J-domain superfamily |
| Superfamily | SSF49493 | HSP40/DnaJ peptide-binding |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 14 | 7 | 23 |
| disruptive | 4 | 20 | 11 | 46 |
| missense | 4 | 20 | 10 | 41 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 33 | 15 | 31 |
| disruptive | 8 | 55 | 50 | 99 |
| missense | 7 | 45 | 42 | 77 |
PlasmoDB Total SNPs: 245
Non-coding: 225 | Synonymous: 14 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 20482550 | Parasite-encoded Hsp40 proteins define novel mobile structures in the cytosol ofthe P. falciparum-infected erythrocyte. | Kulzer S, Rug M, ..., Przyborski JM | 10.1111/j.1462-5822.2010.01477.x |
| 26845441 | The Malarial Exported PFA0660w Is an Hsp40 Co-Chaperone of PfHsp70-x. | Daniyan MO, Boshoff A, ..., Blatch GL | 10.1371/journal.pone.0148517 |
| 30804381 | Cholesterol bound Plasmodium falciparum co-chaperone 'PFA0660w' complexes withmajor virulence factor 'PfEMP1' via chaperone 'PfHsp70-x'. | Behl A, Kumar V, ..., Mishra PC | 10.1038/s41598-019-39217-y |
| 31430682 | Structural insights into the binding mechanism of Plasmodium falciparum exportedHsp40-Hsp70 chaperone pair. | Behl A, Mishra PC | 10.1016/j.compbiolchem.2019.107099 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 34614006 | Co-chaperone involvement in knob biogenesis implicates host-derived chaperones inmalaria virulence. | Diehl M, Roling L, ..., Przyborski JM | 10.1371/journal.ppat.1009969 |
| 38418371 | Plasmodium falciparum J-dot localized J domain protein A8iJp modulates thechaperone activity of human HSPA8 | Sahu W, Bai T, ..., Reddy KS | 10.1002/1873-3468.14836 |
| 38377589 | The Regulatory Effect of Huangshui Polysaccharides on Intestinal Microbiota andMetabolites during In Vitro Fermentation | Li M, Su J, ..., Liang H | 10.1021/acs.jafc.3c08658 |