About Gene List

PF3D7_0201800 (KAHsp40)

Genome location: Pf3D7_02_v3:86,183..89,482(-)

Genome classification: SubtelomericHypervariable

Function and Localization

Product Description: knob associated heat shock protein 40

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020036 Maurer's cleft
Component GO:0005829 cytosol
Component GO:0020030 infected host cell surface knob
Component GO:0005634 nucleus
Function GO:0051087 chaperone binding
Function GO:0005515 protein binding
Function GO:0051082 unfolded protein binding
Process GO:0051085 chaperone cofactor-dependent protein refolding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 0.87 0.62
Trophozoite expressed 0.47 0.77
Schizont expressed 0.10 0.94
Gametocyte not expressed 0.08 0.95

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0201800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.465 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0310000)

  • Relative Growth Rate: 0.90 ± 0.13
  • Confidence: 5.53

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96123

"Best" AlphaFill ligand hit: 1PS (3-pyridinium-1-ylpropane-1-sulfonate, Local RMSD=1.90) with 5AYK (Global RMSD=8.14)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P31689 DnaJ homolog subfamily A member 1 Homo sapiens OrthoDB CHEMBL52073CHEMBL159171

Orthology Information

Ortholog Group (OrthoMCL): OG6_100302

Most Similar Human Ortholog: A0A7I2YQW1

TM-align score: 0.86 | RMSD: 1.20

Seq Identity: 0.31 | Length: 48 / 421

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000132002 DnaJ heat shock protein family (Hsp40) member B1
ENSG00000137094 DnaJ heat shock protein family (Hsp40) member B5
ENSG00000162616 DnaJ heat shock protein family (Hsp40) member B4
ENSG00000187726 DnaJ heat shock protein family (Hsp40) member B13

Protein Information

Protein Length: 421 | Molecular Weight (kDa): 48.283

UniProt ID(s): O96123

PDB ID(s): None

Isoelectric Point: 8.58

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001623 DnaJ domain
InterPro IPR002939 Chaperone DnaJ, C-terminal
InterPro IPR008971 HSP40/DnaJ peptide-binding
InterPro IPR018253 DnaJ domain, conserved site
InterPro IPR036869 Chaperone J-domain superfamily
PFam PF00226 DnaJ domain
PFam PF01556 Chaperone DnaJ, C-terminal
Superfamily SSF46565 Chaperone J-domain superfamily
Superfamily SSF49493 HSP40/DnaJ peptide-binding

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 84

Non-coding: 63 | Synonymous: 13 | Nonsynonymous: 8 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
9804551 Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Tettelin H, ..., Hoffman SL 10.1126/science.282.5391.1126
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
22970262 An exported heat shock protein 40 associates with pathogenesis-related knobs inPlasmodium falciparum infected erythrocytes. Acharya P, Chaubey S, Grover M, Tatu U 10.1371/journal.pone.0044605
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596
31631203 A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. Das D, Krishnan SR, Roy A, Bulusu G 10.1039/c9mo00124g