Product Description: octaprenyl pyrophosphate synthase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.5.1.10 ((2E,6E)-farnesyl diphosphate synthase);2.5.1.32 (15-cis-phytoene synthase);2.5.1.90 (All-trans-octaprenyl-diphosphate synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Component | GO:1990234 | transferase complex |
| Function | GO:0004659 | prenyltransferase activity |
| Process | GO:0016117 | carotenoid biosynthetic process |
| Process | GO:0008299 | isoprenoid biosynthetic process |
| Process | GO:0009657 | plastid organization |
| Process | GO:0006744 | ubiquinone biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.44 | 0.73 |
| Schizont | expressed | 0.15 | 0.90 |
| Gametocyte | expressed | 0.26 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0202700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.144 | MFS: -3.162 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0300800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96130
"Best" AlphaFill ligand hit: PPV (pyrophosphate, Local RMSD=0.05) with 2E90 (Global RMSD=3.19)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.5.1.10 | (2E,6E)-farnesyl diphosphate synthase | APHBPYS04070YS05035taxodoneJDS05119JDS05120celastroltaxodionearenaroneMMV019313bacitracinclodronate... |
| 2.5.1.32 | 15-cis-phytoene synthase | ATPADPAMPGTPNEMNADPHNADP+Tween 20fructosephosphateCGA 103586Chlorophyll... |
| 2.5.1.90 | all-trans-octaprenyl-diphosphate synthase | EDTAnerolidolzoledronatespirohexalineviridicatumtoxin |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100316
Most Similar Human Ortholog: Q5T2R2
TM-align score: 0.79 | RMSD: 3.44
Seq Identity: 0.27 | Length: 365 / 538
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000148459 | decaprenyl diphosphate synthase subunit 1 |
Protein Length: 538 | Molecular Weight (kDa): 63.046
UniProt ID(s): O96130
PDB ID(s): None
Isoelectric Point: 9.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000092 | Polyprenyl synthetase |
| InterPro | IPR008949 | Isoprenoid synthase domain superfamily |
| InterPro | IPR033749 | Polyprenyl synthetase, conserved site |
| PFam | PF00348 | Polyprenyl synthetase |
| Superfamily | SSF48576 | Isoprenoid synthase domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 27 | 18 | 27 |
| disruptive | 6 | 20 | 5 | 42 |
| missense | 6 | 19 | 5 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 50 | 18 | 24 |
| disruptive | 16 | 44 | 32 | 91 |
| missense | 11 | 38 | 26 | 68 |
PlasmoDB Total SNPs: 62
Non-coding: 35 | Synonymous: 21 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15984931 | Identification, molecular cloning and functional characterization of anoctaprenyl pyrophosphate synthase in intra-erythrocytic stages of Plasmodiumfalciparum. | Tonhosolo R, D'Alexandri FL, ..., Katzin AM | 10.1042/BJ20050441 |
| 19203994 | Carotenoid biosynthesis in intraerythrocytic stages of Plasmodium falciparum. | Tonhosolo R, D'Alexandri FL, ..., Katzin AM | 10.1074/jbc.M807464200 |
| 35257658 | Critical role for isoprenoids in apicoplast biogenesis by malaria parasites | Okada M, Rajaram K, ..., Sigala PA | 10.7554/eLife.73208 |