PF3D7_0202900 (DHHC12)

Genome location: Pf3D7_02_v3:138,621..141,266(+)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC12, putative

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0005783	endoplasmic reticulum
Function	GO:0016409	palmitoyltransferase activity
Function	GO:0019706	protein-cysteine S-palmitoyltransferase activity
Process	GO:0018230	peptidyl-L-cysteine S-palmitoylation
Process	GO:0018345	protein palmitoylation
Process	GO:0006612	protein targeting to membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	expressed	0.25	0.85
Schizont	possibly expressed	0.25	0.84
Gametocyte	not expressed	0.11	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0202900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.995 | MFS: -2.624 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A146M427

"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.02) with 6BMM (Global RMSD=9.56)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.225	protein S-acyltransferase	stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103790

Most Similar Human Ortholog: Q9NPG8

TM-align score: 0.87 | RMSD: 2.68

Seq Identity: 0.23 | Length: 277 / 287

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000136247	zinc finger DHHC-type palmitoyltransferase 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	17	7	15
disruptive	6	38	16	32
missense	6	38	16	29

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	25	12	15
disruptive	12	69	28	41
missense	11	62	24	33

PlasmoDB Total SNPs: 2

Non-coding: 2 | Synonymous: 0 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 287 | Molecular Weight (kDa): 34.778

UniProt ID(s): A0A146M427

PDB ID(s): None

Isoelectric Point: 8.75

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001594	Palmitoyltransferase, DHHC domain
PFam	PF01529	Palmitoyltransferase, DHHC domain
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094