Product Description: protein MAK16, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Component | GO:0030687 | preribosome, large subunit precursor |
| Process | GO:0000460 | maturation of 5.8S rRNA |
| Process | GO:0000470 | maturation of LSU-rRNA |
| Process | GO:0042273 | ribosomal large subunit biogenesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 1.18 | 0.49 |
| Trophozoite | expressed | 0.75 | 0.60 |
| Schizont | expressed | 0.15 | 0.91 |
| Gametocyte | expressed | 0.61 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0203700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.391 | MFS: -2.522 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0301500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZWE8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102084
Most Similar Human Ortholog: Q9BXY0
TM-align score: 0.58 | RMSD: 2.90
Seq Identity: 0.46 | Length: 194 / 439
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000198042 | MAK16 homolog |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 19 | 14 | 18 |
| disruptive | 28 | 84 | 49 | 100 |
| missense | 11 | 50 | 31 | 61 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 47 | 14 | 22 |
| disruptive | 41 | 168 | 51 | 113 |
| missense | 15 | 112 | 28 | 55 |
PlasmoDB Total SNPs: 132
Non-coding: 44 | Synonymous: 48 | Nonsynonymous: 40 | Stop Codon: 0
Protein Length: 439 | Molecular Weight (kDa): 51.458
UniProt ID(s): A0A143ZWE8
PDB ID(s): None
Isoelectric Point: 4.21
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR029004 | Ribosomal L28e/Mak16 |
| PFam | PF01778 | Ribosomal L28e/Mak16 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 8552095 | Paf1p, an RNA polymerase II-associated factor in Saccharomyces cerevisiae, mayhave both positive and negative roles in transcription | Shi X, Finkelstein A, ..., Jaehning JA | 10.1128/MCB.16.2.669 |
| 10838225 | SmMAK16, the Schistosoma mansoni homologue of MAK16 from yeast, targets proteintransport to the nucleolus | Milhon JL, Albert TJ, ..., Tracy JW | 10.1016/s0166-6851(00)00221-8 |
| 3332963 | Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation ofthe MAK16 gene and analysis of an adjacent gene essential for growth at lowtemperatures | Wickner RB, Koh TJ, ..., Kaback DB | 10.1002/yea.320030108 |
| 8144453 | Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis ofthe genes in the FUN38-MAK16-SPO7 region | Barton AB, Kaback DB | 10.1128/jb.176.7.1872-1880.1994 |
| 35615984 | Translation initiation factor eIF4G1 modulates assembly of the polypeptide exittunnel region in yeast ribosome biogenesis | Tseng YT, Sung YC, Liu CY, Lo KY | 10.1242/jcs.259540 |
| 6383953 | Temperature-sensitive lethal mutations on yeast chromosome I appear to defineonly a small number of genes | Kaback DB, Oeller PW, ..., Pringle JR | 10.1093/genetics/108.1.67 |
| 10838226 | Plasmodium falciparum erythrocyte membrane protein 1 is anchored to theactin-spectrin junction and knob-associated histidine-rich protein in theerythrocyte skeleton | Oh SS, Voigt S, ..., Chishti AH | 10.1016/s0166-6851(00)00227-9 |
| 16710831 | Mak16p is required for the maturation of 25S and 5.8S rRNAs in the yeastSaccharomyces cerevisiae | Pellett S, Tracy JW | 10.1002/yea.1368 |
| 3551912 | Molecular characterization of chromosomal genes affecting double-stranded RNAreplication in Saccharomyces cerevisiae | Icho T, Lee HS, Sommer SS, Wickner RB | 10.1007/978-1-4684-5251-8_13 |
| 3045810 | Host function of MAK16: G1 arrest by a mak16 mutant of Saccharomyces cerevisiae | Wickner RB | 10.1073/pnas.85.16.6007 |
| 7739558 | Yeast virus propagation depends critically on free 60S ribosomal subunitconcentration | Ohtake Y, Wickner RB | 10.1128/MCB.15.5.2772 |
| 15621414 | Glutathione transferase-like proteins encoded in genomes of yeasts and fungi:insights into evolution of a multifunctional protein superfamily | McGoldrick S, O'Sullivan SM, Sheehan D | 10.1016/j.femsle.2004.10.033 |