Product Description: translocation protein SEC66, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.87 | 0.65 |
| Trophozoite | expressed | 0.67 | 0.62 |
| Schizont | expressed | 0.58 | 0.71 |
| Gametocyte | expressed | 0.59 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0204200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.689 | MFS: -2.589 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0302000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I665
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 8 | 10 | 14 |
| disruptive | 0 | 2 | 2 | 12 |
| missense | 0 | 2 | 2 | 8 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 25 | 14 | 20 |
| disruptive | 1 | 22 | 19 | 60 |
| missense | 0 | 13 | 10 | 31 |
PlasmoDB Total SNPs: 94
Non-coding: 83 | Synonymous: 9 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 276 | Molecular Weight (kDa): 33.414
UniProt ID(s): Q8I665
PDB ID(s): None
Isoelectric Point: 8.79
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR018624 | Translocation protein Sec66 |
| PFam | PF09802 | Translocation protein Sec66 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 15645503 | Genome-wide survey of non-essential genes required for slowed DNAsynthesis-induced filamentous growth in yeast | Kang CM, Jiang YW | 10.1002/yea.1195 |
| 8253836 | A Sec63p-BiP complex from yeast is required for protein translocation in areconstituted proteoliposome | Brodsky JL, Schekman R | 10.1083/jcb.123.6.1355 |
| 32513868 | The ER-associated protease Ste24 prevents N-terminal signal peptide-independenttranslocation into the endoplasmic reticulum in Saccharomyces cerevisiae | Hosomi A, Iida K, ..., Suzuki T | 10.1074/jbc.RA120.012575 |
| 26510791 | Sec66-Dependent Regulation of Yeast Spindle-Pole Body Duplication Through Pom152 | Katta SS, Chen J, ..., Jaspersen SL | 10.1534/genetics.115.178012 |
| 29042437 | Post-translational processing and membrane translocation of the yeast regulatoryMid1 subunit of the Cch1/VGCC/NALCN cation channel family | Iida K, Teng J, ..., Iida H | 10.1074/jbc.M117.810283 |
| 7841522 | Nonlethal sec71-1 and sec72-1 mutations eliminate proteins associated with theSec63p-BiP complex from S. cerevisiae | Fang H, Green N | 10.1091/mbc.5.9.933 |
| 9858571 | The yeast RER2 gene, identified by endoplasmic reticulum protein localizationmutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis | Sato M, Sato K, ..., Nakano A | 10.1128/MCB.19.1.471 |
| 19090812 | Nuclear inner membrane fusion facilitated by yeast Jem1p is required for spindlepole body fusion but not for the first mitotic nuclear division during yeastmating | Nishikawa S, Hirata A, Endo T | 10.1111/j.1365-2443.2008.01236.x |
| 10069807 | Genetic interactions between KAR7/SEC71, KAR8/JEM1, KAR5, and KAR2 during nuclearfusion in Saccharomyces cerevisiae | Brizzio V, Khalfan W, ..., Rose MD | 10.1091/mbc.10.3.609 |
| 8257795 | Structural and functional characterization of Sec66p, a new subunit of thepolypeptide translocation apparatus in the yeast endoplasmic reticulum | Feldheim D, Yoshimura K, Admon A, Schekman R | 10.1091/mbc.4.9.931 |
| 8257794 | Suppression of a sec63 mutation identifies a novel component of the yeastendoplasmic reticulum translocation apparatus | Kurihara T, Silver P | 10.1091/mbc.4.9.919 |
| 14729456 | Subcellular localization and oligomeric structure of the yeast putativestretch-activated Ca2+ channel component Mid1 | Yoshimura H, Tada T, Iida H | 10.1016/j.yexcr.2003.09.020 |