Product Description: aspartate transaminase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.6.1.1 (Aspartate transaminase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004069 | L-aspartate:2-oxoglutarate aminotransferase activity |
| Function | GO:0008483 | transaminase activity |
| Process | GO:0006520 | cellular amino acid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.30 | 0.85 |
| Trophozoite | expressed | 0.54 | 0.68 |
| Schizont | expressed | 0.10 | 0.93 |
| Gametocyte | not expressed | 0.15 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0204500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.736 | MFS: 0.461 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0302300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0302300)
Modification: Disrupted | RMgm-1748
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96142
"Best" AlphaFill ligand hit: OAA (oxaloacetate ion, Local RMSD=0.01) with 4RKD (Global RMSD=2.04)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.6.1.1 | aspartate transaminase | KClProMgCl2malateMaleateadipateTrypsincysteinefumaratephosphatesuccinateglutamate... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P17174 | Aspartate aminotransferase, cytoplasmic | OrthoDB | CHEMBL4238792CHEMBL5203489ZIPRASIDONECHEMBL5182428... |
Ortholog Group (OrthoMCL): OG6_100337
Most Similar Human Ortholog: P17174
TM-align score: 0.95 | RMSD: 1.52
Seq Identity: 0.31 | Length: 401 / 405
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000120053 | glutamic-oxaloacetic transaminase 1 |
| ENSG00000125166 | glutamic-oxaloacetic transaminase 2 |
Protein Length: 405 | Molecular Weight (kDa): 47.005
UniProt ID(s): O96142
PDB ID(s): 3K7Y
Isoelectric Point: 6.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000796 | Aspartate/other aminotransferase |
| InterPro | IPR004838 | Aminotransferases, class-I, pyridoxal-phosphate-binding site |
| InterPro | IPR004839 | Aminotransferase, class I/classII |
| InterPro | IPR015424 | Pyridoxal phosphate-dependent transferase |
| PFam | PF00155 | Aminotransferase, class I/classII |
| Superfamily | SSF53383 | Pyridoxal phosphate-dependent transferase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 31 | 22 | 25 |
| disruptive | 5 | 40 | 19 | 44 |
| missense | 5 | 40 | 19 | 40 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 57 | 18 | 28 |
| disruptive | 12 | 75 | 35 | 102 |
| missense | 11 | 66 | 28 | 78 |
PlasmoDB Total SNPs: 32
Non-coding: 7 | Synonymous: 17 | Nonsynonymous: 8 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30204084 | Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presenceof a divergent cytochrome c oxidase | Seidi A, Muellner-Wong LS, ..., van Dooren GG | 10.7554/eLife.38131 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |