Product Description: 5'-3' exonuclease, putative
SignalP Peptide: MPKRTIYMWLVFLFFFLELAKC
# Transmembrane Domains: 1
EC Numbers: 3.1.11.- (Exodeoxyribonucleases producing 5'-phosphomonoesters.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005634 | nucleus |
| Function | GO:0004534 | 5'-3' exoribonuclease activity |
| Function | GO:0003723 | RNA binding |
| Process | GO:0000956 | nuclear-transcribed mRNA catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.15 | 0.93 |
| Trophozoite | expressed | 0.40 | 0.77 |
| Schizont | not expressed | 0.10 | 0.94 |
| Gametocyte | not expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0204600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.594 | #Insertions: 4
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0302400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0302400)
Modification: Disrupted | RMgm-1749
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96143
"Best" AlphaFill ligand hit: ABH (2(s)-amino-6-boronohexanoic acid, Local RMSD=0.25) with 3MMR (Global RMSD=11.33)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.11.- | Ex: exodeoxyribonuclease V | NTPDNAPNAKClATPEDTAZnCl2sulfatephosphated(GCTGGTGG)d(GCAGGTGG)abc protein... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 62 | 32 | 83 |
| disruptive | 34 | 136 | 70 | 187 |
| missense | 29 | 119 | 64 | 172 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 111 | 54 | 75 |
| disruptive | 69 | 271 | 128 | 258 |
| missense | 49 | 233 | 106 | 207 |
PlasmoDB Total SNPs: 211
Non-coding: 109 | Synonymous: 49 | Nonsynonymous: 53 | Stop Codon: 0
Protein Length: 1188 | Molecular Weight (kDa): 142.895
UniProt ID(s): O96143
PDB ID(s): None
Isoelectric Point: 9.45
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004859 | Putative 5-3 exonuclease |
| InterPro | IPR016494 | 5'-3' exoribonuclease 1 |
| InterPro | IPR027073 | 5'-3' exoribonuclease |
| PFam | PF03159 | Putative 5-3 exonuclease |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |