About Gene List

PF3D7_0204700 (HT1)

Genome location: Pf3D7_02_v3:204,784..207,425(-)

Genome classification: Core

Function and Localization

Product Description: hexose transporter

SignalP Peptide: N/A

# Transmembrane Domains: 12

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0009986 cell surface
Component GO:0020020 food vacuole
Component GO:0005887 integral component of plasma membrane
Component GO:0005634 nucleus
Component GO:0005886 plasma membrane
Function GO:0055056 D-glucose transmembrane transporter activity
Function GO:0005353 fructose transmembrane transporter activity
Function GO:0005355 glucose transmembrane transporter activity
Function GO:0015149 hexose transmembrane transporter activity
Process GO:0015755 fructose transmembrane transport
Process GO:1904659 glucose transmembrane transport
Process GO:0008645 hexose transmembrane transport
Process GO:0009410 response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.24 0.88
Trophozoite expressed 0.31 0.81
Schizont expressed 0.20 0.86
Gametocyte expressed 0.40 0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0204700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.294 | MFS: -2.474 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0302500)

Modification: Tagged | RMgm-1106

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q7KWJ5

"Best" AlphaFill ligand hit: 37X (octyl glucose neopentyl glycol, Local RMSD=0.36) with 5C65 (Global RMSD=4.76)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P11166 Solute carrier family 2, facilitated glucose transporter member 1 HOGENOM, OMA PUG-1US10729691, Example 1US10729691, Example 2US10729691, Example 3...
P19357 Solute carrier family 2, facilitated glucose transporter member 4 HOGENOM CHEMBL4171968CHEMBL4576794CHEMBL4550275CHEMBL4439282...
P11167 Solute carrier family 2, facilitated glucose transporter member 1 Rattus norvegicus HOGENOM, OMA CHEMBL522813
P14672 Solute carrier family 2, facilitated glucose transporter member 4 HOGENOM CHEMBL4171968CHEMBL4793560
P11169 Solute carrier family 2, facilitated glucose transporter member 3 HOGENOM CHEMBL3780717CHEMBL3780534CHEMBL3781994CHEMBL3781827...
P11168 Solute carrier family 2, facilitated glucose transporter member 2 Homo sapiens HOGENOM CHEMBL3780235CHEMBL3781347CHEMBL3781548CHEMBL3781384...
O97467 Putative sugar transporter Plasmodium falciparum OMA, OrthoMCL, OrthoMCL BLAST US10729691, Example 1US10729691, Example 2US10729691, Example 3US10729691, Example 4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100060

Most Similar Human Ortholog: A0A6Q8PFI8

TM-align score: 0.90 | RMSD: 2.40

Seq Identity: 0.27 | Length: 350 / 504

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000059804 solute carrier family 2 member 3
ENSG00000117394 solute carrier family 2 member 1
ENSG00000136856 solute carrier family 2 member 8
ENSG00000142583 solute carrier family 2 member 5
ENSG00000160326 solute carrier family 2 member 6
ENSG00000163581 solute carrier family 2 member 2
ENSG00000173262 solute carrier family 2 member 14
ENSG00000181856 solute carrier family 2 member 4
ENSG00000281165 solute carrier family 2 member 6
ENSG00000288174 solute carrier family 2 member 4

Protein Information

Protein Length: 504 | Molecular Weight (kDa): 56.417

UniProt ID(s): O97467, Q7KWJ5

PDB ID(s): None

Isoelectric Point: 8.75

Protein Domain Annotations:

Source Family ID Description
InterPro IPR003663 Sugar/inositol transporter
InterPro IPR005828 Major facilitator, sugar transporter-like
InterPro IPR005829 Sugar transporter, conserved site
InterPro IPR036259 MFS transporter superfamily
PFam PF00083 Major facilitator, sugar transporter-like
Superfamily SSF103473 MFS transporter superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 10 26 15 35
disruptive 2 11 3 28
missense 2 11 2 21

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 13 63 20 35
disruptive 4 33 30 85
missense 3 23 22 60

PlasmoDB Total SNPs: 138

Non-coding: 117 | Synonymous: 18 | Nonsynonymous: 3 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
9804551 Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Tettelin H, ..., Hoffman SL 10.1126/science.282.5391.1126
10066789 Intraerythrocytic Plasmodium falciparum expresses a high affinity facilitativehexose transporter. Woodrow CJ, Penny JI, Krishna S 10.1074/jbc.274.11.7272
11152613 Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. Krogh A, Larsson B, von Heijne G, Sonnhammer EL 10.1006/jmbi.2000.4315
12238947 Comparative characterization of hexose transporters of Plasmodium knowlesi,Plasmodium yoelii and Toxoplasma gondii highlights functional differences withinthe apicomplexan family. Joet T, Holterman L, ..., Krishna S 10.1042/BJ20021189
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
20132450 Life cycle studies of the hexose transporter of Plasmodium species and geneticvalidation of their essentiality. Slavic K, Straschil U, ..., Krishna S 10.1111/j.1365-2958.2010.07060.x
21136929 Food vacuole proteome of the malarial parasite Plasmodium falciparum. Lamarque M, Tastet C, ..., Dubremetz JF 10.1002/prca.200700112
25894322 Identification of Selective Inhibitors of the Plasmodium falciparum HexoseTransporter PfHT by Screening Focused Libraries of Anti-Malarial Compounds. Ortiz D, Guiguemde WA, ..., Landfear SM 10.1371/journal.pone.0123598
26248369 The Glucose Transporter PfHT1 Is an Antimalarial Target of the HIV ProteaseInhibitor Lopinavir. Kraft TE, Armstrong C, ..., Hruz PW 10.1128/AAC.00899-15
27128092 Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. Swearingen KE, Lindner SE, ..., Sinnis P 10.1371/journal.ppat.1005606
30379851 Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. Briquet S, Ourimi A, ..., Vaquero C 10.1371/journal.pone.0205596
31071153 Identification of druggable small molecule antagonists of the Plasmodiumfalciparum hexose transporter PfHT and assessment of ligand access to the glucosepermeation pathway via FLAG-mediated protein engineering. Heitmeier MR, Hresko RC, ..., Hruz PW 10.1371/journal.pone.0216457
31996846 The molecular basis for sugar import in malaria parasites. Qureshi AA, Suades A, ..., Drew D 10.1038/s41586-020-1963-z
38110471 Characterization of a novel RNAi yeast insecticide that silences mosquito 5-HT1receptor genes Mysore K, Njoroge TM, ..., Duman-Scheel M 10.1038/s41598-023-49799-3
38282613 Functional characterization of Plasmodium vivax hexose transporter 1 Won JY, Mazigo E, Cha SH, Han JH 10.3389/fcimb.2023.1321240