PF3D7_0204800

Genome location: Pf3D7_02_v3:209,149..211,029(-)

Genome classification: Core

Function and Localization

Product Description: 3'-5' exonuclease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.11.1 (Exodeoxyribonuclease I);3.1.11.2 (Exodeoxyribonuclease III)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0008408	3'-5' exonuclease activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.08	0.96
Trophozoite	expressed	0.37	0.77
Schizont	expressed	0.08	0.94
Gametocyte	possibly expressed	0.32	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0204800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.997 | MFS: -2.714 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0302600)

Relative Growth Rate: 1.00 ± 0.07
Confidence: 6.74

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0302600)

Modification: Disrupted | RMgm-1750

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96144

"Best" AlphaFill ligand hit: 5GP (guanosine-5'-monophosphate, Local RMSD=0.95) with 7C4C (Global RMSD=2.55)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.11.1	exodeoxyribonuclease I	NTPEDTAcamptothecinendonuclease CtIPfullerene-oligonucleotide conjugatesingle-stranded DNA-binding protein5'-thiophosphorylated oligonucleotide
3.1.11.2	exodeoxyribonuclease III	DNAPNAEDTAZnCl2p-chloromercuribenzoate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_109411

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	20	6	23
disruptive	15	47	19	55
missense	15	47	19	51

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	34	8	22
disruptive	31	79	42	108
missense	29	68	30	79

PlasmoDB Total SNPs: 102

Non-coding: 59 | Synonymous: 30 | Nonsynonymous: 12 | Stop Codon: 1

Protein Information

Protein Length: 416 | Molecular Weight (kDa): 49.906

UniProt ID(s): O96144

PDB ID(s): None

Isoelectric Point: 9.1

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002562	3'-5' exonuclease domain
InterPro	IPR012337	Ribonuclease H-like superfamily
InterPro	IPR012340	Nucleic acid-binding, OB-fold
InterPro	IPR022967	RNA-binding domain, S1
PFam	PF01612	3'-5' exonuclease domain
Superfamily	SSF50249	Nucleic acid-binding, OB-fold
Superfamily	SSF53098	Ribonuclease H-like superfamily

PMID	Title	Authors	DOI/Link
8944766	Computational method to predict mitochondrially imported proteins and theirtargeting sequences.	Claros MG, Vincens P	10.1111/j.1432-1033.1996.00779.x
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097