Product Description: DNA-directed RNA polymerase II 16 kDa subunit, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000932 | P-body |
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
| Function | GO:0031369 | translation initiation factor binding |
| Process | GO:0031990 | mRNA export from nucleus in response to heat stress |
| Process | GO:0000288 | nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay |
| Process | GO:0045948 | positive regulation of translational initiation |
| Process | GO:0034402 | recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex |
| Process | GO:0006366 | transcription by RNA polymerase II |
| Process | GO:0006367 | transcript |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.03 | 0.98 |
| Trophozoite | expressed | 0.03 | 0.98 |
| Schizont | possibly expressed | 0.00 | 1.00 |
| Gametocyte | expressed | 0.02 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0205500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.409 | MFS: -3.578 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96150
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102742
Most Similar Human Ortholog: O15514
TM-align score: 0.83 | RMSD: 2.39
Seq Identity: 0.28 | Length: 130 / 132
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000144231 | RNA polymerase II subunit D |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 12 | 6 | 6 |
| disruptive | 1 | 0 | 4 | 3 |
| missense | 1 | 0 | 4 | 3 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 17 | 7 | 7 |
| disruptive | 1 | 5 | 9 | 25 |
| missense | 1 | 4 | 7 | 20 |
PlasmoDB Total SNPs: 88
Non-coding: 75 | Synonymous: 13 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 132 | Molecular Weight (kDa): 15.114
UniProt ID(s): O96150
PDB ID(s): None
Isoelectric Point: 6.46
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005574 | RNA polymerase subunit RPB4/RPC9 |
| InterPro | IPR006590 | RNA polymerase Rpb4/RPC9, core |
| InterPro | IPR010997 | HRDC-like superfamily |
| PFam | PF03874 | RNA polymerase subunit RPB4/RPC9 |
| Superfamily | SSF47819 | HRDC-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |