Product Description: adenylosuccinate lyase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.3.2.2 (Adenylosuccinate lyase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005634 | nucleus |
| Function | GO:0004018 | N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity |
| Process | GO:0006163 | purine nucleotide metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.36 | 0.82 |
| Trophozoite | expressed | 0.45 | 0.74 |
| Schizont | expressed | 0.18 | 0.88 |
| Gametocyte | expressed | 0.25 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0206700
Resistome Missense Mutations: D51G
Resistome Compounds with Missense Mutations: DSM267
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -2.994 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0304300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7KWJ4
"Best" AlphaFill ligand hit: N2P (pentane-1,5-diamine, Local RMSD=0.09) with 4NSL (Global RMSD=2.28)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.3.2.2 | adenylosuccinate lyase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100666
Most Similar Human Ortholog: A0A1B0GWF8
TM-align score: 0.87 | RMSD: 2.36
Seq Identity: 0.18 | Length: 383 / 471
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000239900 | adenylosuccinate lyase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 341
Non-coding: 319 | Synonymous: 15 | Nonsynonymous: 6 | Stop Codon: 1
Protein Length: 471 | Molecular Weight (kDa): 55.176
UniProt ID(s): A0A193PBN1, A0A193PCG6, A0A193PCH1, A0A193PD39, E1CD68, E1CD69, E1CDE8, E1CDI1, E1CDY9, O15918, Q7KWJ4
PDB ID(s): None
Isoelectric Point: 6.98
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000362 | Fumarate lyase family |
| InterPro | IPR004769 | Adenylosuccinate lyase |
| InterPro | IPR008948 | L-Aspartase-like |
| InterPro | IPR013539 | Adenylosuccinate lyase PurB, C-terminal |
| InterPro | IPR020557 | Fumarate lyase, conserved site |
| InterPro | IPR022761 | Fumarate lyase, N-terminal |
| PFam | PF00206 | Fumarate lyase, N-terminal |
| PFam | PF08328 | Adenylosuccinate lyase PurB, C-terminal |
| Superfamily | SSF48557 | L-Aspartase-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 19111634 | Elucidation of the substrate specificity, kinetic and catalytic mechanism ofadenylosuccinate lyase from Plasmodium falciparum. | Bulusu V, Srinivasan B, Bopanna MP, Balaram H | 10.1016/j.bbapap.2008.11.021 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |