About Gene List

PF3D7_0207000 (MSP4)

Genome location: Pf3D7_02_v3:276,725..279,258(-)

Genome classification: Core

Function and Localization

Product Description: merozoite surface protein 4

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020036 Maurer's cleft
Component GO:0046658 anchored component of plasma membrane
Component GO:0016021 integral component of membrane
Component GO:0005886 plasma membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring possibly expressed 0.27 0.87
Trophozoite expressed 1.19 0.45
Schizont expressed 2.70 0.09
Gametocyte expressed 0.63 0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0207000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 6 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.251 | MFS: -2.64 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q7KWJ2

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532334

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 5 4 7
disruptive 36 32 10 32
missense 33 28 9 27

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 4 13 8 13
disruptive 52 51 32 60
missense 42 38 22 38

PlasmoDB Total SNPs: 173

Non-coding: 100 | Synonymous: 24 | Nonsynonymous: 49 | Stop Codon: 0

Protein Information

Protein Length: 272 | Molecular Weight (kDa): 30.549

UniProt ID(s): O76244, Q58SV1, Q58SV3, Q58SX7, Q70ID7, Q7KWJ2, Q8T7T5, Q8T7T9, Q8T7U3, Q8T7U6, Q8T7U7

PDB ID(s): None

Isoelectric Point: 4.29

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000742 EGF-like domain
InterPro IPR024731 EGF domain
PFam PF12947 EGF domain
Superfamily SSF57196 N/A

Associated Publications

PMID Title Authors DOI/Link
9804551 Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Tettelin H, ..., Hoffman SL 10.1126/science.282.5391.1126
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
16603573 Identification and stoichiometry of glycosylphosphatidylinositol-anchoredmembrane proteins of the human malaria parasite Plasmodium falciparum. Gilson PR, Nebl T, ..., Crabb BS 10.1074/mcp.M600035-MCP200
17944453 Identifying merozoite surface protein 4 and merozoite surface protein 7Plasmodium falciparum protein family members specifically binding to humanerythrocytes suggests a new malarial parasite-redundant survival mechanism. Garcia Y, Puentes A, ..., Patarroyo ME 10.1021/jm070773z
29042203 Association of antibodies to Plasmodium falciparum merozoite surface protein-4with protection against clinical malaria. Perraut R, Varela ML, ..., Mercereau-Puijalon O 10.1016/j.vaccine.2017.10.012
31631203 A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. Das D, Krishnan SR, Roy A, Bulusu G 10.1039/c9mo00124g