Product Description: serine repeat antigen 8
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005615 | extracellular space |
| Component | GO:0005764 | lysosome |
| Function | GO:0004197 | cysteine-type endopeptidase activity |
| Process | GO:0051603 | proteolysis involved in cellular protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.00 | 1.00 |
| Trophozoite | not expressed | 0.00 | 1.00 |
| Schizont | not expressed | 0.02 | 0.99 |
| Gametocyte | not expressed | 0.02 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0207300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.998 | MFS: -2.593 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0305100)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0304700)
Modification: Disrupted | RMgm-5064
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZWJ1
"Best" AlphaFill ligand hit: I3C (5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, Local RMSD=0.23) with 6X42 (Global RMSD=4.87)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 17 | 6 | 34 |
| disruptive | 42 | 23 | 9 | 48 |
| missense | 41 | 20 | 9 | 42 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 25 | 18 | 42 |
| disruptive | 44 | 47 | 29 | 56 |
| missense | 43 | 38 | 27 | 40 |
PlasmoDB Total SNPs: 82
Non-coding: 27 | Synonymous: 17 | Nonsynonymous: 38 | Stop Codon: 0
Protein Length: 679 | Molecular Weight (kDa): 79.357
UniProt ID(s): A0A0E3VL24, A0A0E3VL33, A0A0E4B1U9, A0A0E4B266, A0A0E4B382, A0A0E4B3A7, A0A0E4B3G3, A0A0E4FLE6, A0A143ZWJ1
PDB ID(s): None
Isoelectric Point: 7.92
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000169 | Cysteine peptidase, cysteine active site |
| InterPro | IPR000668 | Peptidase C1A, papain C-terminal |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF00112 | Peptidase C1A, papain C-terminal |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 17893128 | Evidence for a common role for the serine-type Plasmodium falciparum serinerepeat antigen proteases: implications for vaccine and drug design | McCoubrie JE, Miller SK, ..., Crabb BS | 10.1128/IAI.00405-07 |
| 17609844 | Phylogeny and evolution of the SERA multigene family in the genus Plasmodium | Arisue N, Hirai M, ..., Horii T | 10.1007/s00239-006-0253-1 |