Product Description: serine repeat antigen 6
SignalP Peptide: MICPIFFLYIINVLFTQYFIKCEG
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005615 | extracellular space |
| Component | GO:0005764 | lysosome |
| Component | GO:0005634 | nucleus |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0004197 | cysteine-type endopeptidase activity |
| Function | GO:0008234 | cysteine-type peptidase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0035891 | exit from host cell |
| Process | GO:0002377 | immunoglobulin production |
| Process | GO:0006508 | proteolysis |
| Process | GO:0051603 | proteolysis involved in cellular protein catabolic process |
| Process | GO:0050776 | regulation of immune response |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.07 | 0.97 |
| Trophozoite | expressed | 0.86 | 0.63 |
| Schizont | expressed | 1.07 | 0.57 |
| Gametocyte | not expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0207500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.252 | MFS: -2.761 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0305100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0304900)
Modification: Tagged | RMgm-272
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9TY96
"Best" AlphaFill ligand hit: E64 (n-[n-[1-hydroxycarboxyethyl-carbonyl]leucylamino-butyl]-guanidine, Local RMSD=0.21) with 3BPF (Global RMSD=6.58)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 44 | 28 | 57 |
| disruptive | 39 | 86 | 46 | 101 |
| missense | 34 | 79 | 44 | 87 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 72 | 40 | 57 |
| disruptive | 59 | 137 | 69 | 154 |
| missense | 46 | 124 | 61 | 116 |
PlasmoDB Total SNPs: 224
Non-coding: 170 | Synonymous: 19 | Nonsynonymous: 35 | Stop Codon: 0
Protein Length: 1031 | Molecular Weight (kDa): 118.836
UniProt ID(s): A0A0E3VL22, A0A0E3VL29, A0A0E4B250, A0A0E4B386, A0A0E4B3C7, A0A0E4B3H8, A0A0E4FHY3, Q26015, Q26017, Q9TY96
PDB ID(s): None
Isoelectric Point: 6.21
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000169 | Cysteine peptidase, cysteine active site |
| InterPro | IPR000668 | Peptidase C1A, papain C-terminal |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF00112 | Peptidase C1A, papain C-terminal |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12228245 | A subset of Plasmodium falciparum SERA genes are expressed and appear to play animportant role in the erythrocytic cycle. | Miller SK, Good RT, ..., Crabb BS | 10.1074/jbc.M206974200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 22984267 | Proteolytic activation of the essential parasitophorous vacuole cysteine proteaseSERA6 accompanies malaria parasite egress from its host erythrocyte. | Ruecker A, Shea M, ..., Blackman MJ | 10.1074/jbc.M112.400820 |
| 29459732 | A protease cascade regulates release of the human malaria parasite Plasmodiumfalciparum from host red blood cells. | Thomas JA, Tan MSY, ..., Blackman MJ | 10.1038/s41564-018-0111-0 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33932049 | Autocatalytic activation of a malarial egress protease is druggable and requiresa protein cofactor. | Tan MSY, Koussis K, ..., Blackman MJ | 10.15252/embj.2020107226 |
| 17609844 | Phylogeny and evolution of the SERA multigene family in the genus Plasmodium | Arisue N, Hirai M, ..., Horii T | 10.1007/s00239-006-0253-1 |
| 1654826 | Genetic analysis of yeast iso-1-cytochrome c structural requirements: suppressionof Gly6 replacements by an Asn52----Ile replacement | Berroteran RW, Hampsey M | 10.1016/0003-9861(91)90193-m |
| 17893128 | Evidence for a common role for the serine-type Plasmodium falciparum serinerepeat antigen proteases: implications for vaccine and drug design | McCoubrie JE, Miller SK, ..., Crabb BS | 10.1128/IAI.00405-07 |
| 15306658 | The serine repeat antigen (SERA) gene family phylogeny in Plasmodium: the impactof GC content and reconciliation of gene and species trees | Bourgon R, Delorenzi M, ..., Speed TP | 10.1093/molbev/msh228 |
| 21660657 | Falcipains and other cysteine proteases of malaria parasites | Rosenthal PJ | 10.1007/978-1-4419-8414-2_3 |
| 13679369 | Enzymic, phylogenetic, and structural characterization of the unusual papain-likeprotease domain of Plasmodium falciparum SERA5 | Hodder AN, Drew DR, ..., Crabb BS | 10.1074/jbc.M306755200 |
| 32252804 | Characteristic features of the SERA multigene family in the malaria parasite | Arisue N, Palacpac NMQ, Tougan T, Horii T | 10.1186/s13071-020-04044-y |