Product Description: serine repeat antigen 5
SignalP Peptide: MKSYISLFFILCVIFNKNVIKCTG
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005615 | extracellular space |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005764 | lysosome |
| Component | GO:0005634 | nucleus |
| Component | GO:0020004 | symbiont-containing vacuolar space |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0004197 | cysteine-type endopeptidase activity |
| Function | GO:0008234 | cysteine-type peptidase activity |
| Function | GO:0019900 | kinase binding |
| Function | GO:0008233 | peptidase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0035891 | exit from host cell |
| Process | GO:0002377 | immunoglobulin production |
| Process | GO:0006508 | proteolysis |
| Process | GO:0051603 | proteolysis involved in cellular protein catabolic process |
| Process | GO:0050776 | regulation of immune response |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.30 | 0.86 |
| Trophozoite | expressed | 1.32 | 0.59 |
| Schizont | expressed | 2.72 | 0.36 |
| Gametocyte | expressed | 0.13 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0207600
Resistome Missense Mutations: D434N
Resistome Compounds with Missense Mutations: MMV982556
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.2 | MFS: -2.737 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0305100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9TY95
"Best" AlphaFill ligand hit: I3C (5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, Local RMSD=0.42) with 6X42 (Global RMSD=1.82)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 32 | 15 | 33 |
| disruptive | 109 | 109 | 35 | 122 |
| missense | 49 | 47 | 18 | 69 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 29 | 49 | 26 | 40 |
| disruptive | 139 | 149 | 60 | 152 |
| missense | 55 | 78 | 40 | 77 |
PlasmoDB Total SNPs: 306
Non-coding: 64 | Synonymous: 90 | Nonsynonymous: 151 | Stop Codon: 1
Protein Length: 997 | Molecular Weight (kDa): 111.768
UniProt ID(s): A0A0E3VL27, A0A0E4B1V1, A0A0E4B234, A0A0E4B242, A0A0E4B3A9, A0A0E4B3K2, A0A0E4FHX1, A0A348AW43, I0IZ29, I0IZF8, Q26016, Q9NIF4, Q9TY95
Isoelectric Point: 5.06
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000668 | Peptidase C1A, papain C-terminal |
| InterPro | IPR025660 | Cysteine peptidase, histidine active site |
| InterPro | IPR025661 | Cysteine peptidase, asparagine active site |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF00112 | Peptidase C1A, papain C-terminal |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12228245 | A subset of Plasmodium falciparum SERA genes are expressed and appear to play animportant role in the erythrocytic cycle. | Miller SK, Good RT, ..., Crabb BS | 10.1074/jbc.M206974200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21203490 | Compartmentation of redox metabolism in malaria parasites. | Kehr S, Sturm N, ..., Becker K | 10.1371/journal.ppat.1001242 |
| 21630256 | Antibodies reactive to Plasmodium falciparum serine repeat antigen in childrenwith Burkitt lymphoma from Ghana. | Guech-Ongey M, Yagi M, ..., Mbulaiteye SM | 10.1002/ijc.26203 |
| 24769454 | Synthetic peptides derived from the C-terminal 6kDa region of Plasmodiumfalciparum SERA5 inhibit the enzyme activity and malaria parasite development. | Kanodia S, Kumar G, ..., Malhotra P | 10.1016/j.bbagen.2014.04.013 |
| 25599609 | Plasmodium falciparum SERA5 plays a non-enzymatic role in the malarial asexualblood-stage lifecycle. | Stallmach R, Kavishwar M, ..., Blackman MJ | 10.1111/mmi.12941 |
| 28202027 | A novel Pfs38 protein complex on the surface of Plasmodium falciparum blood-stagemerozoites. | Paul G, Deshmukh A, ..., Malhotra P | 10.1186/s12936-017-1716-0 |
| 28683142 | The Plasmodium falciparum pseudoprotease SERA5 regulates the kinetics andefficiency of malaria parasite egress from host erythrocytes. | Collins CR, Hackett F, ..., Blackman MJ | 10.1371/journal.ppat.1006453 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 29567995 | Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of HostVitronectin to P47 Fragment of SERA5. | Tougan T, Edula JR, ..., Horii T | 10.1038/s41598-018-23194-9 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31148576 | Plasmodium pseudo-Tyrosine Kinase-like binds PP1 and SERA5 and is exported tohost erythrocytes. | Gnangnon B, Freville A, ..., Pierrot C | 10.1038/s41598-019-44542-3 |
| 32955133 | Structure of the Plasmodium falciparum PfSERA5 pseudo-zymogen. | Smith NA, Clarke OB, ..., Smith BJ | 10.1002/pro.3956 |
| 28808204 | Safety, immunogenicity, and cross-species protection of a plasmid DNA encodingPlasmodium falciparum SERA5 polypeptide, microbial epitopes and chemokine genesin mice and olive baboons | Onkoba N, M Mumo R, ..., G Egwang T | 10.7555/JBR.31.20160025 |
| 37908361 | Safety and immunogenicity of BK-SE36/CpG malaria vaccine in healthy Burkinabeadults and children: a phase 1b randomised, controlled, double-blinded, agede-escalation trial | Ouedraogo A, Bougouma EC, ..., Sirima SB | 10.3389/fimmu.2023.1267372 |
| 27343834 | Immunogenicity and protection from malaria infection in BK-SE36 vaccinatedvolunteers in Uganda is not influenced by HLA-DRB1 alleles | Tougan T, Ito K, ..., Horii T | 10.1016/j.parint.2016.06.012 |
| 29716996 | Calcium-dependent phosphorylation of Plasmodium falciparum serine repeat antigen5 triggers merozoite egress | Iyer GR, Singh S, ..., Malhotra P | 10.1074/jbc.RA117.001540 |
| 29760216 | Plasmodium falciparum MSP3 Exists in a Complex on the Merozoite Surface andGenerates Antibody Response during Natural Infection | Deshmukh A, Chourasia BK, ..., Malhotra P | 10.1128/IAI.00067-18 |
| 33012605 | First-in-human randomised trial and follow-up study of Plasmodium falciparumblood-stage malaria vaccine BK-SE36 with CpG-ODN(K3) | Ezoe S, Palacpac NMQ, ..., Horii T | 10.1016/j.vaccine.2020.09.056 |
| 30483336 | Genetic Diversity in C-terminal of SERA5 Gene in the Blood Stage of HumanIsolates of Plasmodium vivax in Sistan and Baluchistan, Iran | Abolghazi A, Heidari A, ..., Shojaee S | https://pubmed.ncbi.nlm.nih.gov/30483336/ |
| 33918470 | Assessment of Mixed Plasmodium falciparum sera5 Infection in Endemic BurkittLymphoma: A Case-Control Study in Malawi | Arisue N, Chagaluka G, ..., Mbulaiteye SM | 10.3390/cancers13071692 |
| 26725489 | In silico approach to ascertain the calcium dependent role of Plasmodiumfalciparum SERA5 | Rahul CN, Shiva Krishna K, Pawar AP, Rajesh V | 10.1080/07391102.2015.1129988 |
| 33032613 | Age-dependent carriage of alleles and haplotypes of Plasmodium falciparum sera5,eba-175, and csp in a region of intense malaria transmission in Uganda | Agwang C, Erume J, ..., Egwang TG | 10.1186/s12936-020-03432-0 |
| 36993981 | Plasmodium falciparum infection coinciding with the malaria vaccine candidateBK-SE36 administration interferes with the immune responses in Burkinabechildren | Tiono AB, Palacpac NMQ, ..., Sirima SB | 10.3389/fimmu.2023.1119820 |
| 32252804 | Characteristic features of the SERA multigene family in the malaria parasite | Arisue N, Palacpac NMQ, Tougan T, Horii T | 10.1186/s13071-020-04044-y |
| 36590593 | African-specific polymorphisms in Plasmodium falciparum serine repeat antigen 5in Uganda and Burkina Faso clinical samples do not interfere with antibodyresponse to BK-SE36 vaccination | Arisue N, Palacpac NMQ, ..., Horii T | 10.3389/fcimb.2022.1058081 |
| 28662682 | Pooled-DNA sequencing identifies genomic regions of selection in Nigerianisolates of Plasmodium falciparum | Oyebola KM, Idowu ET, ..., Amambua-Ngwa A | 10.1186/s13071-017-2260-z |
| 30579868 | Associations between IgG reactivity to Plasmodium falciparum erythrocyte membraneprotein 1 (PfEMP1) antigens and Burkitt lymphoma in Ghana and Uganda case-controlstudies | Derkach A, Otim I, ..., Mbulaiteye SM | 10.1016/j.ebiom.2018.12.020 |