Product Description: serine repeat antigen 4
SignalP Peptide: MKIHIFLIATIYVLFSEKLIKWTTA
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005615 | extracellular space |
| Component | GO:0005764 | lysosome |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0004197 | cysteine-type endopeptidase activity |
| Function | GO:0008234 | cysteine-type peptidase activity |
| Process | GO:0002377 | immunoglobulin production |
| Process | GO:0006508 | proteolysis |
| Process | GO:0051603 | proteolysis involved in cellular protein catabolic process |
| Process | GO:0050776 | regulation of immune response |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.35 | 0.82 |
| Schizont | expressed | 0.73 | 0.67 |
| Gametocyte | not expressed | 0.01 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0207700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.947 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0305100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96164
"Best" AlphaFill ligand hit: I3C (5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, Local RMSD=0.52) with 6X42 (Global RMSD=2.00)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 26 | 13 | 38 |
| disruptive | 45 | 71 | 42 | 100 |
| missense | 37 | 63 | 35 | 89 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 35 | 27 | 38 |
| disruptive | 56 | 122 | 61 | 130 |
| missense | 47 | 106 | 54 | 111 |
PlasmoDB Total SNPs: 209
Non-coding: 56 | Synonymous: 87 | Nonsynonymous: 66 | Stop Codon: 0
Protein Length: 962 | Molecular Weight (kDa): 108.681
UniProt ID(s): A0A0E3VL25, A0A0E3VL34, A0A0E4B1U4, A0A0E4B1X4, A0A0E4B262, A0A0E4B3A0, A0A0E4B3F5, A0A0E4B3J3, A0A0E4FLE3, O96164
PDB ID(s): None
Isoelectric Point: 5.51
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000668 | Peptidase C1A, papain C-terminal |
| InterPro | IPR025661 | Cysteine peptidase, asparagine active site |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF00112 | Peptidase C1A, papain C-terminal |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12228245 | A subset of Plasmodium falciparum SERA genes are expressed and appear to play animportant role in the erythrocytic cycle. | Miller SK, Good RT, ..., Crabb BS | 10.1074/jbc.M206974200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23485360 | Plasmodium vivax: C-terminal diversity in the blood stage SERA genes from Indianfield isolates | Rahul CN, Shiva Krishna K, ..., Rajesh V | 10.1016/j.exppara.2013.02.004 |
| 18591232 | Inhibition of malaria parasite development by a cyclic peptide that targets thevital parasite protein SERA5 | Fairlie WD, Spurck TP, ..., Hodder AN | 10.1128/IAI.00278-08 |
| 24218484 | Sequential processing of merozoite surface proteins during and after erythrocyteinvasion by Plasmodium falciparum | Boyle MJ, Langer C, ..., Beeson JG | 10.1128/IAI.00866-13 |
| 28683142 | The Plasmodium falciparum pseudoprotease SERA5 regulates the kinetics andefficiency of malaria parasite egress from host erythrocytes | Collins CR, Hackett F, ..., Blackman MJ | 10.1371/journal.ppat.1006453 |
| 17893128 | Evidence for a common role for the serine-type Plasmodium falciparum serinerepeat antigen proteases: implications for vaccine and drug design | McCoubrie JE, Miller SK, ..., Crabb BS | 10.1128/IAI.00405-07 |