Product Description: serine repeat antigen 3
SignalP Peptide: MKFSISLFLILCVLFCKNDIKC
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005615 | extracellular space |
| Component | GO:0005764 | lysosome |
| Function | GO:0004197 | cysteine-type endopeptidase activity |
| Function | GO:0008234 | cysteine-type peptidase activity |
| Process | GO:0002377 | immunoglobulin production |
| Process | GO:0006508 | proteolysis |
| Process | GO:0051603 | proteolysis involved in cellular protein catabolic process |
| Process | GO:0050776 | regulation of immune response |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.27 | 0.85 |
| Schizont | expressed | 0.73 | 0.63 |
| Gametocyte | possibly expressed | 0.01 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0207800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.533 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0305100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96165
"Best" AlphaFill ligand hit: I3C (5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, Local RMSD=0.45) with 6X42 (Global RMSD=2.08)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 36 | 20 | 46 |
| disruptive | 17 | 109 | 47 | 113 |
| missense | 17 | 108 | 47 | 105 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 59 | 35 | 49 |
| disruptive | 31 | 167 | 81 | 154 |
| missense | 31 | 160 | 75 | 138 |
PlasmoDB Total SNPs: 159
Non-coding: 108 | Synonymous: 19 | Nonsynonymous: 32 | Stop Codon: 0
Protein Length: 930 | Molecular Weight (kDa): 105.423
UniProt ID(s): A0A0E3VL32, A0A0E4B246, A0A0E4B3H0, A0A0E4FID6, I0IZG7, I0IZZ0, I0J017, I0J020, I0J029, O96165
PDB ID(s): None
Isoelectric Point: 7.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000668 | Peptidase C1A, papain C-terminal |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF00112 | Peptidase C1A, papain C-terminal |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12228245 | A subset of Plasmodium falciparum SERA genes are expressed and appear to play animportant role in the erythrocytic cycle. | Miller SK, Good RT, ..., Crabb BS | 10.1074/jbc.M206974200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 7891737 | Analysis of stage-specific transcripts of the Plasmodium falciparum serine repeatantigen (SERA) gene and transcription from the SERA locus | Fox BA, Bzik DJ | 10.1016/0166-6851(94)00162-6 |
| 37595939 | Genetic diversity and in silico analysis of Plasmodium knowlesi Serine RepeatAntigen (SERA) 3 antigen 2 in Malaysia | Tan JH, Ding HX, Fong MY, Lau YL | 10.1016/j.meegid.2023.105490 |
| 30941868 | The Plasmodium berghei serine protease PbSUB1 plays an important role in malegamete egress | Pace T, Grasso F, ..., Olivieri A | 10.1111/cmi.13028 |
| 32252804 | Characteristic features of the SERA multigene family in the malaria parasite | Arisue N, Palacpac NMQ, Tougan T, Horii T | 10.1186/s13071-020-04044-y |
| 18419771 | Expression and processing of Plasmodium berghei SERA3 during liver stages | Schmidt-Christensen A, Sturm A, Horstmann S, Heussler VT | 10.1111/j.1462-5822.2008.01162.x |
| 12244052 | Serine repeat antigen (SERA5) is predominantly expressed among the SERA multigenefamily of Plasmodium falciparum, and the acquired antibody titers correlate withserum inhibition of the parasite growth | Aoki S, Li J, ..., Horii T | 10.1074/jbc.M207145200 |