Product Description: serine repeat antigen 1
SignalP Peptide: MKFFIPYVYIICVIFIINVIRTRG
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005615 | extracellular space |
| Component | GO:0005764 | lysosome |
| Function | GO:0004197 | cysteine-type endopeptidase activity |
| Function | GO:0008234 | cysteine-type peptidase activity |
| Process | GO:0002377 | immunoglobulin production |
| Process | GO:0006508 | proteolysis |
| Process | GO:0051603 | proteolysis involved in cellular protein catabolic process |
| Process | GO:0050776 | regulation of immune response |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.99 |
| Trophozoite | not expressed | 0.19 | 0.89 |
| Schizont | possibly expressed | 0.76 | 0.60 |
| Gametocyte | not expressed | 0.32 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0208000
Resistome Missense Mutations: N184D
Resistome Compounds with Missense Mutations: BRD73842
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.98 | MFS: -2.772 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0305100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96167
"Best" AlphaFill ligand hit: E64 (n-[n-[1-hydroxycarboxyethyl-carbonyl]leucylamino-butyl]-guanidine, Local RMSD=0.32) with 3H7D (Global RMSD=9.99)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 43 | 31 | 60 |
| disruptive | 17 | 100 | 68 | 153 |
| missense | 17 | 97 | 64 | 148 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 90 | 37 | 63 |
| disruptive | 44 | 196 | 107 | 257 |
| missense | 38 | 181 | 94 | 212 |
PlasmoDB Total SNPs: 177
Non-coding: 103 | Synonymous: 48 | Nonsynonymous: 26 | Stop Codon: 0
Protein Length: 994 | Molecular Weight (kDa): 115.496
UniProt ID(s): A0A0E3VL28, A0A0E4B1T9, A0A0E4B265, A0A0E4B3I5, A0A0E4FHY6, A0A0E4FJA4, I0IYU3, I0IYU9, I0IYV8, I0IYZ9, O96167
PDB ID(s): None
Isoelectric Point: 8.06
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000668 | Peptidase C1A, papain C-terminal |
| InterPro | IPR025661 | Cysteine peptidase, asparagine active site |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF00112 | Peptidase C1A, papain C-terminal |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12228245 | A subset of Plasmodium falciparum SERA genes are expressed and appear to play animportant role in the erythrocytic cycle. | Miller SK, Good RT, ..., Crabb BS | 10.1074/jbc.M206974200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 17609844 | Phylogeny and evolution of the SERA multigene family in the genus Plasmodium | Arisue N, Hirai M, ..., Horii T | 10.1007/s00239-006-0253-1 |
| 31052291 | Targeting the Serine Pathway: A Promising Approach against Tuberculosis | Haufroid M, Wouters J | 10.3390/ph12020066 |
| 29684399 | A Cas9 transgenic Plasmodium yoelii parasite for efficient gene editing | Qian P, Wang X, ..., Yuan J | 10.1016/j.molbiopara.2018.04.003 |
| 17893128 | Evidence for a common role for the serine-type Plasmodium falciparum serinerepeat antigen proteases: implications for vaccine and drug design | McCoubrie JE, Miller SK, ..., Crabb BS | 10.1128/IAI.00405-07 |
| 14963770 | Transcription status of vaccine candidate genes of Plasmodium falciparum duringthe hepatic phase of its life cycle | Bodescot M, Silvie O, ..., Mazier D | 10.1007/s00436-003-1061-9 |