PF3D7_0208900 (P230p)

Genome location: Pf3D7_02_v3:360,828..368,354(+)

Genome classification: Core

Function and Localization

Product Description: 6-cysteine protein P230p

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0046658	anchored component of plasma membrane
Component	GO:0009986	cell surface
Component	GO:0005737	cytoplasm
Process	GO:0044409	entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.06	0.97
Trophozoite	possibly expressed	0.10	0.95
Schizont	possibly expressed	0.05	0.97
Gametocyte	expressed	0.56	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0208900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.524 | #Insertions: 12

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1107600)

Relative Growth Rate: 0.98 ± 0.06
Confidence: 7.03

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0306000)

Modification: Disrupted | RMgm-4559

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96175

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=5.37) with 4JWJ (Global RMSD=15.85)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_160720

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	107	57	179
disruptive	69	218	123	333
missense	57	201	116	299

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	31	205	106	168
disruptive	127	442	208	419
missense	90	379	188	333

PlasmoDB Total SNPs: 201

Non-coding: 1 | Synonymous: 119 | Nonsynonymous: 81 | Stop Codon: 0

Protein Information

Protein Length: 2508 | Molecular Weight (kDa): 293.647

UniProt ID(s): A0A144A0J8, O96175

PDB ID(s): 7JUM

Isoelectric Point: 7.74

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR010884	6-Cysteine (6-Cys) domain
PFam	PF07422	6-Cysteine (6-Cys) domain
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12106866	Male-specific expression of the paralog of malaria transmission-blocking targetantigen Pfs230, PfB0400w.	Eksi S, Williamson KC	10.1016/s0166-6851(02)00091-9
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
30297725	The Plasmodium falciparum male gametocyte protein P230p, a paralog of P230, isvital for ookinete formation and mosquito transmission.	Marin-Mogollon C, van de Vegte-Bolmer M, ..., Khan SM	10.1038/s41598-018-33236-x
20386715	Three members of the 6-cys protein family of Plasmodium play a role in gametefertility	van Dijk MR, van Schaijk BC, ..., Janse CJ	10.1371/journal.ppat.1000853
31053746	Author Correction: The Plasmodium falciparum male gametocyte protein P230p, aparalog of P230, is vital for ookinete formation and mosquito transmission	Marin-Mogollon C, van de Vegte-Bolmer M, ..., Khan SM	10.1038/s41598-019-43505-y
25012929	Plasmodium yoelii vitamin B5 pantothenate transporter candidate is essential forparasite transmission to the mosquito	Hart RJ, Lawres L, ..., Aly AS	10.1038/srep05665