Product Description: 6-cysteine protein P230
SignalP Peptide: MKKIITLKNLFLIILVYIFS
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0046658 | anchored component of plasma membrane |
| Component | GO:0009986 | cell surface |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
| Process | GO:0009405 | obsolete pathogenesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.98 |
| Trophozoite | not expressed | 0.05 | 0.98 |
| Schizont | not expressed | 0.04 | 0.98 |
| Gametocyte | possibly expressed | 2.39 | 0.24 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0209000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.851 | #Insertions: 12
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0306100)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0306100)
Modification: Disrupted | RMgm-4559
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 92 | 56 | 152 |
| disruptive | 121 | 177 | 111 | 287 |
| missense | 91 | 143 | 97 | 248 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 32 | 185 | 80 | 190 |
| disruptive | 176 | 338 | 187 | 383 |
| missense | 117 | 271 | 163 | 295 |
PlasmoDB Total SNPs: 353
Non-coding: 111 | Synonymous: 118 | Nonsynonymous: 124 | Stop Codon: 0
Protein Length: 3135 | Molecular Weight (kDa): 363.218
UniProt ID(s): A0A143ZY09, P68874, Q25994, Q9GTK4, Q9U6T4, Q9U6T5
PDB ID(s): 7USR, 7USS, 7UST, 7USV
Isoelectric Point: 5.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR010884 | 6-Cysteine (6-Cys) domain |
| PFam | PF07422 | 6-Cysteine (6-Cys) domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 1380124 | Further characterization of interactions between gamete surface antigens ofPlasmodium falciparum. | Kumar N, Wizel B | 10.1016/0166-6851(92)90013-a |
| 8479460 | Cloning and expression of the gene for Plasmodium falciparumtransmission-blocking target antigen, Pfs230. | Williamson KC, Criscio MD, Kaslow DC | 10.1016/0166-6851(93)90058-6 |
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16879650 | Malaria transmission-blocking antigen, Pfs230, mediates human red blood cellbinding to exflagellating male parasites and oocyst production. | Eksi S, Czesny B, ..., Williamson KC | 10.1111/j.1365-2958.2006.05284.x |
| 17950739 | PfCCp proteins of Plasmodium falciparum: gametocyte-specific expression and rolein complement-mediated inhibition of exflagellation. | Scholz SM, Simon N, ..., Pradel G | 10.1016/j.ijpara.2007.08.009 |
| 21124765 | The dynamics of naturally acquired immune responses to Plasmodium falciparumsexual stage antigens Pfs230 & Pfs48/45 in a low endemic area in Tanzania. | Bousema T, Roeffen W, ..., Drakeley C | 10.1371/journal.pone.0014114 |
| 26537493 | A WD40-repeat protein unique to malaria parasites associates with adhesionprotein complexes and is crucial for blood stage progeny. | von Bohl A, Kuehn A, ..., Pradel G | 10.1186/s12936-015-0967-x |
| 27432885 | Structural and Immunological Characterization of Recombinant 6-Cysteine Domainsof the Plasmodium falciparum Sexual Stage Protein Pfs230. | MacDonald NJ, Nguyen V, ..., Narum DL | 10.1074/jbc.M116.732305 |
| 32709983 | Structure and function of a malaria transmission blocking vaccine targetingPfs230 and Pfs230-Pfs48/45 proteins. | Singh K, Burkhardt M, ..., Narum DL | 10.1038/s42003-020-01123-9 |
| 36253843 | Genetic diversity in the transmission-blocking vaccine candidate Plasmodium vivaxgametocyte protein Pvs230 from the China-Myanmar border area and central Myanmar | Zhao X, Hu Y, ..., Cao Y | 10.1186/s13071-022-05523-0 |